Restriction map for
pF1KB6699
(Linear) (Six-base) MAPSORT of: KIBB6699.seq Check: 4912 from: 1 to: 912
KIBB6699.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 737 912
Size: 737 175
AflIII A'CryG_T
Cuts at: 0 467 912
Size: 467 445
AgeI A'CCGG_T
Cuts at: 0 712 912
Size: 712 200
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 296 912
Size: 296 616
ApaI G_GGCC'C
Cuts at: 0 409 524 565 912
Size: 409 115 41 347
Fragments arranged by size:
409 347 115 41
AvaI C'yCGr_G
Cuts at: 0 633 912
Size: 633 279
BanII G_rGCy'C
Cuts at: 0 409 460 524 565 912
Size: 409 51 64 41 347
Fragments arranged by size:
409 347 64 51 41
Bme1580I G_kGCm'C
Cuts at: 0 409 524 565 912
Size: 409 115 41 347
Fragments arranged by size:
409 347 115 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 335 912
Size: 335 577
BsaI GGTCTCn'nnnn_
Cuts at: 0 243 912
Size: 243 669
BsaWI w'CCGG_w
Cuts at: 0 712 912
Size: 712 200
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 74 104 416 446 912
Size: 74 30 312 30 466
Fragments arranged by size:
466 312 74 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 769 912
Size: 769 143
BseYI C'CCAG_C
Cuts at: 0 398 912
Size: 398 514
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 912
Size: 163 749
BsiHKAI G_wGCw'C
Cuts at: 0 170 227 460 808 912
Size: 170 57 233 348 104
Fragments arranged by size:
348 233 170 104 57
Bsp1286I G_dGCh'C
Cuts at: 0 170 227 409 460 524 565 808 912
Size: 170 57 182 51 64 41 243 104
Fragments arranged by size:
243 182 170 104 64 57 51 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 737 912
Size: 737 175
BsrDI GCAATG_nn'
Cuts at: 0 148 912
Size: 148 764
BsrFI r'CCGG_y
Cuts at: 0 712 912
Size: 712 200
BstEII G'GTnAC_C
Cuts at: 0 715 912
Size: 715 197
BstXI CCAn_nnnn'nTGG
Cuts at: 0 417 912
Size: 417 495
BtgI C'CryG_G
Cuts at: 0 410 573 912
Size: 410 163 339
Fragments arranged by size:
410 339 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 781 912
Size: 781 131
BtsI GCAGTG_nn'
Cuts at: 0 643 912
Size: 643 269
EaeI y'GGCC_r
Cuts at: 0 58 912
Size: 58 854
EarI CTCTTCn'nnn_
Cuts at: 0 119 787 912
Size: 119 668 125
Fragments arranged by size:
668 125 119
EcoICRI GAG'CTC
Cuts at: 0 458 912
Size: 458 454
EcoO109I rG'GnC_Cy
Cuts at: 0 53 106 287 406 520 562 746 912
Size: 53 53 181 119 114 42 184 166
Fragments arranged by size:
184 181 166 119 114 53 53 42
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 267 912
Size: 267 645
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 223 255 912
Size: 223 32 657
Fragments arranged by size:
657 223 32
HincII GTy'rAC
Cuts at: 0 531 912
Size: 531 381
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 833 912
Size: 833 79
MscI TGG'CCA
Cuts at: 0 60 912
Size: 60 852
MslI CAynn'nnrTG
Cuts at: 0 230 731 912
Size: 230 501 181
Fragments arranged by size:
501 230 181
NspI r_CATG'y
Cuts at: 0 471 736 912
Size: 471 265 176
Fragments arranged by size:
471 265 176
PciI A'CATG_T
Cuts at: 0 467 912
Size: 467 445
PfoI T'CCnGG_A
Cuts at: 0 451 912
Size: 451 461
PpuMI rG'GwC_Cy
Cuts at: 0 106 287 746 912
Size: 106 181 459 166
Fragments arranged by size:
459 181 166 106
PspOMI G'GGCC_C
Cuts at: 0 405 520 561 912
Size: 405 115 41 351
Fragments arranged by size:
405 351 115 41
PspXI vC'TCGA_Gb
Cuts at: 0 633 912
Size: 633 279
PstI C_TGCA'G
Cuts at: 0 50 448 519 849 912
Size: 50 398 71 330 63
Fragments arranged by size:
398 330 71 63 50
SacI G_AGCT'C
Cuts at: 0 460 912
Size: 460 452
SbfI CC_TGCA'GG
Cuts at: 0 849 912
Size: 849 63
SfcI C'TryA_G
Cuts at: 0 46 291 444 515 585 845 912
Size: 46 245 153 71 70 260 67
Fragments arranged by size:
260 245 153 71 70 67 46
SmlI C'TyrA_G
Cuts at: 0 633 912
Size: 633 279
StyI C'CwwG_G
Cuts at: 0 242 524 912
Size: 242 282 388
Fragments arranged by size:
388 282 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 116 250 586 705 912
Size: 116 134 336 119 207
Fragments arranged by size:
336 207 134 119 116
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 416 448 912
Size: 416 32 464
Fragments arranged by size:
464 416 32
Tth111I GACn'n_nGTC
Cuts at: 0 358 438 912
Size: 358 80 474
Fragments arranged by size:
474 358 80
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 348 912
Size: 348 564
XhoI C'TCGA_G
Cuts at: 0 633 912
Size: 633 279
XmnI GAAnn'nnTTC
Cuts at: 0 84 669 912
Size: 84 585 243
Fragments arranged by size:
585 243 84
Enzymes that do cut:
AarI AflIII AgeI AlfI ApaI AvaI BanII Bme1580I
BmrI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrDI BsrFI BstEII BstXI BtgI BtgZI
BtsI EaeI EarI EcoICRI EcoO109I FalI Hin4I HincII
MmeI MscI MslI NspI PciI PfoI PpuMI PspOMI
PspXI PstI SacI SbfI SfcI SmlI StyI TaqII
TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AhdI
AleI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstYI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EagI EciI Eco57MI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PmeI PmlI PpiI PshAI PsiI PsrI PvuI PvuII
RsrII SacII SalI SanDI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI XbaI XmaI ZraI