(Linear) (Six-base) MAPSORT of: KIBB6701.seq  Check: 469  from: 1  to: 186



KIBB6701.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      19      52     186 
   Size:         19      33     134

  Fragments arranged by size: 

                 134      33      19
 
AlwNI CAG_nnn'CTG

Cuts at:      0      56     179     186 
   Size:         56     123       7

  Fragments arranged by size: 

                 123      56       7
 
BglI GCCn_nnn'nGGC

Cuts at:      0     119     186 
   Size:        119      67
 
Bme1580I G_kGCm'C

Cuts at:      0     138     186 
   Size:        138      48
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      24     186 
   Size:         24     162
 
Bsp1286I G_dGCh'C

Cuts at:      0     138     186 
   Size:        138      48
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      19      52     186 
   Size:         19      33     134

  Fragments arranged by size: 

                 134      33      19
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      71      86     179     186 
   Size:         71      15      93       7

  Fragments arranged by size: 

                  93      71      15       7
 
EarI CTCTTCn'nnn_

Cuts at:      0      88     186 
   Size:         88      98
 
MspA1I CmG'CkG

Cuts at:      0      25     176     186 
   Size:         25     151      10

  Fragments arranged by size: 

                 151      25      10
 
PasI CC'CwG_GG

Cuts at:      0     138     186 
   Size:        138      48
 
PstI C_TGCA'G

Cuts at:      0      30     186 
   Size:         30     156
 
PvuII CAG'CTG

Cuts at:      0     176     186 
   Size:        176      10
 
SapI GCTCTTCn'nnn_

Cuts at:      0      88     186 
   Size:         88      98
 
SfcI C'TryA_G

Cuts at:      0      26     186 
   Size:         26     160
 

 Enzymes that do cut:

     AarI    AlwNI     BglI Bme1580I     BsgI Bsp1286I    BspMI   BstAPI
     EarI   MspA1I     PasI     PstI    PvuII     SapI     SfcI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI