(Linear) (Six-base) MAPSORT of: KIBB6702.seq Check: 6863 from: 1 to: 384 KIBB6702.orf With 182 enzymes: SgfI * June 26, 2008 10:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 51 355 384 Size: 51 304 29 Fragments arranged by size: 304 51 29 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 313 384 Size: 313 71 AlwNI CAG_nnn'CTG Cuts at: 0 66 384 Size: 66 318 ApoI r'AATT_y Cuts at: 0 212 384 Size: 212 172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 255 289 384 Size: 255 34 95 Fragments arranged by size: 255 95 34 BsaI GGTCTCn'nnnn_ Cuts at: 0 36 384 Size: 36 348 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 51 355 384 Size: 51 304 29 Fragments arranged by size: 304 51 29 BsrDI GCAATG_nn' Cuts at: 0 79 261 384 Size: 79 182 123 Fragments arranged by size: 182 123 79 BsrGI T'GTAC_A Cuts at: 0 348 384 Size: 348 36 BstAPI GCAn_nnn'nTGC Cuts at: 0 66 384 Size: 66 318 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 313 384 Size: 313 71 EcoRI G'AATT_C Cuts at: 0 212 384 Size: 212 172 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 147 384 Size: 147 237 MspA1I CmG'CkG Cuts at: 0 185 384 Size: 185 199 NspI r_CATG'y Cuts at: 0 192 384 Size: 192 192 PflMI CCAn_nnn'nTGG Cuts at: 0 237 384 Size: 237 147 TatI w'GTAC_w Cuts at: 0 348 384 Size: 348 36 XbaI T'CTAG_A Cuts at: 0 156 384 Size: 156 228 Enzymes that do cut: AarI AcuI AlwNI ApoI BcgI BsaI BspMI BsrDI BsrGI BstAPI Eco57MI EcoRI FalI MspA1I NspI PflMI TatI XbaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TstI Tth111I XcmI XhoI XmaI XmnI ZraI