(Linear) (Six-base) MAPSORT of: KIBB6703.seq  Check: 9668  from: 1  to: 219



KIBB6703.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

Acc65I G'GTAC_C

Cuts at:      0     106     219 
   Size:        106     113
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     219 
   Size:        151      68
 
AflII C'TTAA_G

Cuts at:      0      79     219 
   Size:         79     140
 
AflIII A'CryG_T

Cuts at:      0     161     219 
   Size:        161      58
 
AhdI GACnn_n'nnGTC

Cuts at:      0     162     219 
   Size:        162      57
 
BanI G'GyrC_C

Cuts at:      0     106     219 
   Size:        106     113
 
BsaAI yAC'GTr

Cuts at:      0     162     219 
   Size:        162      57
 
BsiEI CG_ry'CG

Cuts at:      0      24     219 
   Size:         24     195
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     219 
   Size:        151      68
 
EcoO109I rG'GnC_Cy

Cuts at:      0      75     219 
   Size:         75     144
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     148     180     219 
   Size:        148      32      39

  Fragments arranged by size: 

                 148      39      32
 
KpnI G_GTAC'C

Cuts at:      0     110     219 
   Size:        110     109
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     219 
   Size:         94     125
 
PmlI CAC'GTG

Cuts at:      0     162     219 
   Size:        162      57
 
PpuMI rG'GwC_Cy

Cuts at:      0      75     219 
   Size:         75     144
 
SmlI C'TyrA_G

Cuts at:      0      79     219 
   Size:         79     140
 

 Enzymes that do cut:

   Acc65I     AcuI    AflII   AflIII     AhdI     BanI    BsaAI    BsiEI
  Eco57MI EcoO109I    Hin4I     KpnI     MmeI     PmlI    PpuMI     SmlI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI     AgeI     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI