(Linear) (Six-base) MAPSORT of: KIBB6704.seq  Check: 8841  from: 1  to: 384



KIBB6704.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     103     384 
   Size:        103     281
 
AlwNI CAG_nnn'CTG

Cuts at:      0      13     208     384 
   Size:         13     195     176

  Fragments arranged by size: 

                 195     176      13
 
BbvCI CC'TCA_GC

Cuts at:      0      91     318     384 
   Size:         91     227      66

  Fragments arranged by size: 

                 227      91      66
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     308     384 
   Size:        308      76
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      77     384 
   Size:         77     307
 
Bpu10I CC'TnA_GC

Cuts at:      0      91     318     384 
   Size:         91     227      66

  Fragments arranged by size: 

                 227      91      66
 
BsaWI w'CCGG_w

Cuts at:      0     230     384 
   Size:        230     154
 
BspEI T'CCGG_A

Cuts at:      0     230     384 
   Size:        230     154
 
BsrFI r'CCGG_y

Cuts at:      0      43     384 
   Size:         43     341
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      72     384 
   Size:         72     312
 
BstYI r'GATC_y

Cuts at:      0     357     384 
   Size:        357      27
 
BtsI GCAGTG_nn'

Cuts at:      0      14     384 
   Size:         14     370
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     298     330     384 
   Size:        298      32      54

  Fragments arranged by size: 

                 298      54      32
 
PstI C_TGCA'G

Cuts at:      0     296     384 
   Size:        296      88
 
SfcI C'TryA_G

Cuts at:      0     292     341     384 
   Size:        292      49      43

  Fragments arranged by size: 

                 292      49      43
 
TatI w'GTAC_w

Cuts at:      0     119     328     384 
   Size:        119     209      56

  Fragments arranged by size: 

                 209     119      56
 

 Enzymes that do cut:

     AlfI    AlwNI    BbvCI    BciVI     BmrI   Bpu10I    BsaWI    BspEI
    BsrFI    BstXI    BstYI     BtsI    Hin4I     PstI     SfcI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI