(Linear) (Six-base) MAPSORT of: KIBB6705.seq  Check: 6484  from: 1  to: 228



KIBB6705.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AflIII A'CryG_T

Cuts at:      0      75     228 
   Size:         75     153
 
ApoI r'AATT_y

Cuts at:      0      31     228 
   Size:         31     197
 
AvaI C'yCGr_G

Cuts at:      0      19     228 
   Size:         19     209
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      26     228 
   Size:         26     202
 
NspI r_CATG'y

Cuts at:      0      79     228 
   Size:         79     149
 
PciI A'CATG_T

Cuts at:      0      75     228 
   Size:         75     153
 
SpeI A'CTAG_T

Cuts at:      0     148     228 
   Size:        148      80
 
SspI AAT'ATT

Cuts at:      0      89     168     228 
   Size:         89      79      60

  Fragments arranged by size: 

                  89      79      60
 
StyI C'CwwG_G

Cuts at:      0      81     215     228 
   Size:         81     134      13

  Fragments arranged by size: 

                 134      81      13
 

 Enzymes that do cut:

   AflIII     ApoI     AvaI     BplI     NspI     PciI     SpeI     SspI
     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI