(Linear) (Six-base) MAPSORT of: KIBB6706.seq  Check: 9283  from: 1  to: 387



KIBB6706.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

ApoI r'AATT_y

Cuts at:      0     336     387 
   Size:        336      51
 
AseI AT'TA_AT

Cuts at:      0     199     387 
   Size:        199     188
 
BanII G_rGCy'C

Cuts at:      0     140     387 
   Size:        140     247
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     387 
   Size:        387
 
BbvCI CC'TCA_GC

Cuts at:      0     182     387 
   Size:        182     205
 
BglII A'GATC_T

Cuts at:      0     275     387 
   Size:        275     112
 
Bpu10I CC'TnA_GC

Cuts at:      0     182     387 
   Size:        182     205
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     165     387 
   Size:        165     222
 
Bsp1286I G_dGCh'C

Cuts at:      0     140     387 
   Size:        140     247
 
BsrDI GCAATG_nn'

Cuts at:      0     368     387 
   Size:        368      19
 
BssSI C'ACGA_G

Cuts at:      0      10     387 
   Size:         10     377
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     153     387 
   Size:        153     234
 
BstYI r'GATC_y

Cuts at:      0     275     387 
   Size:        275     112
 
DraI TTT'AAA

Cuts at:      0     335     387 
   Size:        335      52
 
EaeI y'GGCC_r

Cuts at:      0     270     387 
   Size:        270     117
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     250     387 
   Size:        250     137
 
MfeI C'AATT_G

Cuts at:      0     227     387 
   Size:        227     160
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     291     387 
   Size:        119     172      96

  Fragments arranged by size: 

                 172     119      96
 
MscI TGG'CCA

Cuts at:      0     272     387 
   Size:        272     115
 
MspA1I CmG'CkG

Cuts at:      0      48     387 
   Size:         48     339
 
PstI C_TGCA'G

Cuts at:      0     158     387 
   Size:        158     229
 
PvuII CAG'CTG

Cuts at:      0      48     387 
   Size:         48     339
 
SfcI C'TryA_G

Cuts at:      0     154     387 
   Size:        154     233
 
StyI C'CwwG_G

Cuts at:      0      17     387 
   Size:         17     370
 

 Enzymes that do cut:

     ApoI     AseI    BanII     BbsI    BbvCI    BglII   Bpu10I     BsaI
 Bsp1286I    BsrDI    BssSI   BstAPI    BstYI     DraI     EaeI    EcoNI
     MfeI     MmeI     MscI   MspA1I     PstI    PvuII     SfcI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MluI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI