(Linear) (Six-base) MAPSORT of: KIBB6707.seq  Check: 5125  from: 1  to: 237



KIBB6707.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     237 
   Size:        137     100
 
AhdI GACnn_n'nnGTC

Cuts at:      0     139     237 
   Size:        139      98
 
BglII A'GATC_T

Cuts at:      0      14     237 
   Size:         14     223
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     123     153     237 
   Size:        123      30      84

  Fragments arranged by size: 

                 123      84      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     136     144     237 
   Size:        136       8      93

  Fragments arranged by size: 

                 136      93       8
 
BseYI C'CCAG_C

Cuts at:      0     158     237 
   Size:        158      79
 
BspHI T'CATG_A

Cuts at:      0     177     237 
   Size:        177      60
 
BsrFI r'CCGG_y

Cuts at:      0      56     237 
   Size:         56     181
 
BstYI r'GATC_y

Cuts at:      0      14     237 
   Size:         14     223
 
EarI CTCTTCn'nnn_

Cuts at:      0     113     221     237 
   Size:        113     108      16

  Fragments arranged by size: 

                 113     108      16
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     237 
   Size:        137     100
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     125     157     237 
   Size:        125      32      80

  Fragments arranged by size: 

                 125      80      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     168     237 
   Size:        168      69
 
NspI r_CATG'y

Cuts at:      0      64     237 
   Size:         64     173
 
ScaI AGT'ACT

Cuts at:      0      34     237 
   Size:         34     203
 
TatI w'GTAC_w

Cuts at:      0      32     237 
   Size:         32     205
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     123     155     237 
   Size:        123      32      82

  Fragments arranged by size: 

                 123      82      32
 

 Enzymes that do cut:

     AcuI     AhdI    BglII    BsaXI    BseRI    BseYI    BspHI    BsrFI
    BstYI     EarI  Eco57MI    Hin4I     MmeI     NspI     ScaI     TatI
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI