(Linear) (Six-base) MAPSORT of: KIBB6708.seq  Check: 3706  from: 1  to: 387



KIBB6708.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     194     387 
   Size:        194     193
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      92     124     387 
   Size:         92      32     263

  Fragments arranged by size: 

                 263      92      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      48     378     387 
   Size:         48     330       9

  Fragments arranged by size: 

                 330      48       9
 
BanI G'GyrC_C

Cuts at:      0     214     366     387 
   Size:        214     152      21

  Fragments arranged by size: 

                 214     152      21
 
BanII G_rGCy'C

Cuts at:      0      57     354     387 
   Size:         57     297      33

  Fragments arranged by size: 

                 297      57      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     230     387 
   Size:        230     157
 
BglI GCCn_nnn'nGGC

Cuts at:      0      43      52      68     387 
   Size:         43       9      16     319

  Fragments arranged by size: 

                 319      43      16       9
 
Bme1580I G_kGCm'C

Cuts at:      0     371     387 
   Size:        371      16
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      59     143     387 
   Size:         59      84     244

  Fragments arranged by size: 

                 244      84      59
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     387 
   Size:        119     268
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      92     122     387 
   Size:         92      30     265

  Fragments arranged by size: 

                 265      92      30
 
BseYI C'CCAG_C

Cuts at:      0     329     371     387 
   Size:        329      42      16

  Fragments arranged by size: 

                 329      42      16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     298     387 
   Size:        298      89
 
BsiEI CG_ry'CG

Cuts at:      0      77     387 
   Size:         77     310
 
Bsp1286I G_dGCh'C

Cuts at:      0      57     354     371     387 
   Size:         57     297      17      16

  Fragments arranged by size: 

                 297      57      17      16
 
Bsu36I CC'TnA_GG

Cuts at:      0      49     387 
   Size:         49     338
 
BtgI C'CryG_G

Cuts at:      0      89     387 
   Size:         89     298
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      94     348     387 
   Size:         94     254      39

  Fragments arranged by size: 

                 254      94      39
 
EaeI y'GGCC_r

Cuts at:      0      35      74     387 
   Size:         35      39     313

  Fragments arranged by size: 

                 313      39      35
 
EagI C'GGCC_G

Cuts at:      0      74     387 
   Size:         74     313
 
EarI CTCTTCn'nnn_

Cuts at:      0     116     275     387 
   Size:        116     159     112

  Fragments arranged by size: 

                 159     116     112
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     194     387 
   Size:        119      75     193

  Fragments arranged by size: 

                 193     119      75
 
EcoO109I rG'GnC_Cy

Cuts at:      0      45     387 
   Size:         45     342
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     275     387 
   Size:        119     156     112

  Fragments arranged by size: 

                 156     119     112
 
MspA1I CmG'CkG

Cuts at:      0     344     378     387 
   Size:        344      34       9

  Fragments arranged by size: 

                 344      34       9
 
NcoI C'CATG_G

Cuts at:      0      89     387 
   Size:         89     298
 
NotI GC'GGCC_GC

Cuts at:      0      74     387 
   Size:         74     313
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     378     387 
   Size:        378       9
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      92     124     387 
   Size:         92      32     263

  Fragments arranged by size: 

                 263      92      32
 
PvuII CAG'CTG

Cuts at:      0     378     387 
   Size:        378       9
 
SapI GCTCTTCn'nnn_

Cuts at:      0     116     387 
   Size:        116     271
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      43     387 
   Size:         43     344
 
StyI C'CwwG_G

Cuts at:      0      89     387 
   Size:         89     298
 
TatI w'GTAC_w

Cuts at:      0     189     387 
   Size:        189     198
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI     BanI    BanII     BbsI     BglI Bme1580I
     BmrI     BpmI    BsaXI    BseYI     BsgI    BsiEI Bsp1286I   Bsu36I
     BtgI    BtgZI     EaeI     EagI     EarI  Eco57MI EcoO109I     MmeI
   MspA1I     NcoI     NotI    PflMI     PpiI    PvuII     SapI     SfiI
     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI
     NdeI   NgoMIV     NheI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI