(Linear) (Six-base) MAPSORT of: KIBB6710.seq  Check: 2686  from: 1  to: 390



KIBB6710.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

ApoI r'AATT_y

Cuts at:      0     145     390 
   Size:        145     245
 
BbvCI CC'TCA_GC

Cuts at:      0     318     390 
   Size:        318      72
 
BclI T'GATC_A

Cuts at:      0     221     390 
   Size:        221     169
 
BmgBI CAC'GTC

Cuts at:      0     240     390 
   Size:        240     150
 
Bpu10I CC'TnA_GC

Cuts at:      0     318     390 
   Size:        318      72
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      16     390 
   Size:         16     374
 
BsaWI w'CCGG_w

Cuts at:      0     104     390 
   Size:        104     286
 
BseYI C'CCAG_C

Cuts at:      0     172     390 
   Size:        172     218
 
BsiHKAI G_wGCw'C

Cuts at:      0      91     390 
   Size:         91     299
 
Bsp1286I G_dGCh'C

Cuts at:      0      91     390 
   Size:         91     299
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     172     390 
   Size:        172     218
 
FspI TGC'GCA

Cuts at:      0       7     390 
   Size:          7     383
 
FspAI rTGC'GCAy

Cuts at:      0       7     390 
   Size:          7     383
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     356     388     390 
   Size:        356      32       2

  Fragments arranged by size: 

                 356      32       2
 
MslI CAynn'nnrTG

Cuts at:      0     118     390 
   Size:        118     272
 
MspA1I CmG'CkG

Cuts at:      0     322     390 
   Size:        322      68
 
PvuII CAG'CTG

Cuts at:      0     322     390 
   Size:        322      68
 
SmlI C'TyrA_G

Cuts at:      0      31     390 
   Size:         31     359
 
TatI w'GTAC_w

Cuts at:      0     306     390 
   Size:        306      84
 

 Enzymes that do cut:

     ApoI    BbvCI     BclI    BmgBI   Bpu10I    BpuEI    BsaWI    BseYI
  BsiHKAI Bsp1286I    BstXI     FspI    FspAI    Hin4I     MslI   MspA1I
    PvuII     SmlI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI