(Linear) (Six-base) MAPSORT of: KIBB6712.seq  Check: 9933  from: 1  to: 396



KIBB6712.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

BamHI G'GATC_C

Cuts at:      0     219     396 
   Size:        219     177
 
BanI G'GyrC_C

Cuts at:      0      54     396 
   Size:         54     342
 
Bme1580I G_kGCm'C

Cuts at:      0     268     396 
   Size:        268     128
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     117     262     396 
   Size:        117     145     134

  Fragments arranged by size: 

                 145     134     117
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     116     396 
   Size:        116     280
 
Bsp1286I G_dGCh'C

Cuts at:      0     268     396 
   Size:        268     128
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     212     396 
   Size:        212     184
 
BstYI r'GATC_y

Cuts at:      0     219     396 
   Size:        219     177
 
BtsI GCAGTG_nn'

Cuts at:      0     172     396 
   Size:        172     224
 
EaeI y'GGCC_r

Cuts at:      0     279     324     396 
   Size:        279      45      72

  Fragments arranged by size: 

                 279      72      45
 
EarI CTCTTCn'nnn_

Cuts at:      0      22     175     396 
   Size:         22     153     221

  Fragments arranged by size: 

                 221     153      22
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     116     396 
   Size:        116     280
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     357     396 
   Size:        357      39
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     174     206     396 
   Size:        174      32     190

  Fragments arranged by size: 

                 190     174      32
 
HincII GTy'rAC

Cuts at:      0     228     396 
   Size:        228     168
 
MscI TGG'CCA

Cuts at:      0     281     326     396 
   Size:        281      45      70

  Fragments arranged by size: 

                 281      70      45
 
MslI CAynn'nnrTG

Cuts at:      0     358     396 
   Size:        358      38
 
MspA1I CmG'CkG

Cuts at:      0      11     396 
   Size:         11     385
 
NspI r_CATG'y

Cuts at:      0     363     396 
   Size:        363      33
 
PasI CC'CwG_GG

Cuts at:      0      42     396 
   Size:         42     354
 
StyI C'CwwG_G

Cuts at:      0      58     396 
   Size:         58     338
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     313     345     396 
   Size:        313      32      51

  Fragments arranged by size: 

                 313      51      32
 

 Enzymes that do cut:

    BamHI     BanI Bme1580I     BmrI     BpmI Bsp1286I   BstAPI    BstYI
     BtsI     EaeI     EarI  Eco57MI     FalI    Hin4I   HincII     MscI
     MslI   MspA1I     NspI     PasI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI