(Linear) (Six-base) MAPSORT of: KIBB6714.seq  Check: 6029  from: 1  to: 399



KIBB6714.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     370     399 
   Size:        370      29
 
AlwNI CAG_nnn'CTG

Cuts at:      0     348     399 
   Size:        348      51
 
ApoI r'AATT_y

Cuts at:      0     281     399 
   Size:        281     118
 
AvaI C'yCGr_G

Cuts at:      0     179     374     399 
   Size:        179     195      25

  Fragments arranged by size: 

                 195     179      25
 
BanI G'GyrC_C

Cuts at:      0      16     399 
   Size:         16     383
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     279     399 
   Size:        279     120
 
BclI T'GATC_A

Cuts at:      0     105     399 
   Size:        105     294
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     399 
   Size:        244     155
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     194     224     399 
   Size:        194      30     175

  Fragments arranged by size: 

                 194     175      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     215     399 
   Size:        215     184
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     399 
   Size:        361      38
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     370     399 
   Size:        244     126      29

  Fragments arranged by size: 

                 244     126      29
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      31     399 
   Size:         31     368
 
EcoO109I rG'GnC_Cy

Cuts at:      0     345     399 
   Size:        345      54
 
PshAI GACnn'nnGTC

Cuts at:      0     359     399 
   Size:        359      40
 
PspXI vC'TCGA_Gb

Cuts at:      0     179     374     399 
   Size:        179     195      25

  Fragments arranged by size: 

                 195     179      25
 
SmlI C'TyrA_G

Cuts at:      0     179     374     399 
   Size:        179     195      25

  Fragments arranged by size: 

                 195     179      25
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     371     399 
   Size:        371      28
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     194     226     399 
   Size:        194      32     173

  Fragments arranged by size: 

                 194     173      32
 
XbaI T'CTAG_A

Cuts at:      0     285     399 
   Size:        285     114
 
XhoI C'TCGA_G

Cuts at:      0     179     374     399 
   Size:        179     195      25

  Fragments arranged by size: 

                 195     179      25
 
XmnI GAAnn'nnTTC

Cuts at:      0     281     399 
   Size:        281     118
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     AvaI     BanI    BciVI     BclI     BpmI
    BsaXI    BseRI     BsgI  Eco57MI    EcoNI EcoO109I    PshAI    PspXI
     SmlI    TaqII     TstI     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI  Tth111I     XcmI     XmaI     ZraI