Restriction map for pF1KB6715
 (Linear) (Six-base) MAPSORT of: KIBB6715.seq  Check: 6842  from: 1  to: 243



KIBB6715.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     169     243 
   Size:        169      74
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      22     166     243 
   Size:         22     144      77

  Fragments arranged by size: 

                 144      77      22
 
BsaWI w'CCGG_w

Cuts at:      0      25     243 
   Size:         25     218
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     106     236     239     243 
   Size:        106     130       3       4

  Fragments arranged by size: 

                 130     106       4       3
 
DraIII CAC_nnn'GTG

Cuts at:      0     207     243 
   Size:        207      36
 
MspA1I CmG'CkG

Cuts at:      0     169     243 
   Size:        169      74
 
PvuII CAG'CTG

Cuts at:      0     169     243 
   Size:        169      74
 
SexAI A'CCwGG_T

Cuts at:      0     203     243 
   Size:        203      40
 
SfcI C'TryA_G

Cuts at:      0      56     132     243 
   Size:         56      76     111

  Fragments arranged by size: 

                 111      76      56
 

 Enzymes that do cut:

    AlwNI     BbsI    BsaWI    BseRI   DraIII   MspA1I    PvuII    SexAI
     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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