(Linear) (Six-base) MAPSORT of: KIBB6715.seq Check: 6842 from: 1 to: 243 KIBB6715.orf With 182 enzymes: SgfI * April 24, 2009 13:06 .. AlwNI CAG_nnn'CTG Cuts at: 0 169 243 Size: 169 74 BbsI GAAGACnn'nnnn_ Cuts at: 0 22 166 243 Size: 22 144 77 Fragments arranged by size: 144 77 22 BsaWI w'CCGG_w Cuts at: 0 25 243 Size: 25 218 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 106 236 239 243 Size: 106 130 3 4 Fragments arranged by size: 130 106 4 3 DraIII CAC_nnn'GTG Cuts at: 0 207 243 Size: 207 36 MspA1I CmG'CkG Cuts at: 0 169 243 Size: 169 74 PvuII CAG'CTG Cuts at: 0 169 243 Size: 169 74 SexAI A'CCwGG_T Cuts at: 0 203 243 Size: 203 40 SfcI C'TryA_G Cuts at: 0 56 132 243 Size: 56 76 111 Fragments arranged by size: 111 76 56 Enzymes that do cut: AlwNI BbsI BsaWI BseRI DraIII MspA1I PvuII SexAI SfcI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI