(Linear) (Six-base) MAPSORT of: KIBB6717.seq  Check: 7547  from: 1  to: 252



KIBB6717.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

BamHI G'GATC_C

Cuts at:      0      17     252 
   Size:         17     235
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     252 
   Size:        203      49
 
Bpu10I CC'TnA_GC

Cuts at:      0     153     252 
   Size:        153      99
 
BsaWI w'CCGG_w

Cuts at:      0      20     252 
   Size:         20     232
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     109     252 
   Size:        109     143
 
BspEI T'CCGG_A

Cuts at:      0      20     252 
   Size:         20     232
 
BsrFI r'CCGG_y

Cuts at:      0     244     252 
   Size:        244       8
 
BstYI r'GATC_y

Cuts at:      0      17     252 
   Size:         17     235
 
Bsu36I CC'TnA_GG

Cuts at:      0      82     252 
   Size:         82     170
 
BtgI C'CryG_G

Cuts at:      0     115     252 
   Size:        115     137
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     152     187     252 
   Size:        152      35      65

  Fragments arranged by size: 

                 152      65      35
 
EaeI y'GGCC_r

Cuts at:      0       5     252 
   Size:          5     247
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     252 
   Size:        203      49
 
HincII GTy'rAC

Cuts at:      0     160     252 
   Size:        160      92
 
MscI TGG'CCA

Cuts at:      0       7     252 
   Size:          7     245
 
MspA1I CmG'CkG

Cuts at:      0      15     252 
   Size:         15     237
 
NcoI C'CATG_G

Cuts at:      0     115     252 
   Size:        115     137
 
PfoI T'CCnGG_A

Cuts at:      0     180     252 
   Size:        180      72
 
StyI C'CwwG_G

Cuts at:      0     115     252 
   Size:        115     137
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      70     252 
   Size:         70     182
 

 Enzymes that do cut:

    BamHI     BpmI   Bpu10I    BsaWI    BseRI    BspEI    BsrFI    BstYI
   Bsu36I     BtgI    CspCI     EaeI  Eco57MI   HincII     MscI   MspA1I
     NcoI     PfoI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I    BtgZI     BtsI     ClaI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI