(Linear) (Six-base) MAPSORT of: KIBB6719.seq  Check: 9843  from: 1  to: 252



KIBB6719.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     219     252 
   Size:        219      33
 
BanII G_rGCy'C

Cuts at:      0      82     252 
   Size:         82     170
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      17     252 
   Size:         17     235
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      55     252 
   Size:         55     197
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     252 
   Size:         94     158
 
Bsp1286I G_dGCh'C

Cuts at:      0      82     252 
   Size:         82     170
 
BstYI r'GATC_y

Cuts at:      0      15     252 
   Size:         15     237
 
BtgI C'CryG_G

Cuts at:      0     131     252 
   Size:        131     121
 
EarI CTCTTCn'nnn_

Cuts at:      0      32      40     252 
   Size:         32       8     212

  Fragments arranged by size: 

                 212      32       8
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      17     219     252 
   Size:         17     202      33

  Fragments arranged by size: 

                 202      33      17
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     200     237     252 
   Size:        200      37      15

  Fragments arranged by size: 

                 200      37      15
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     232     252 
   Size:        232      20
 
MslI CAynn'nnrTG

Cuts at:      0     100     252 
   Size:        100     152
 
SfcI C'TryA_G

Cuts at:      0     197     252 
   Size:        197      55
 

 Enzymes that do cut:

     AcuI    BanII     BpmI    BseRI     BsgI Bsp1286I    BstYI     BtgI
     EarI  Eco57MI    EcoNI     FalI     MslI     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI