(Linear) (Six-base) MAPSORT of: KIBB6725.seq  Check: 4692  from: 1  to: 276



KIBB6725.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     276 
   Size:         87     189
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     233     276 
   Size:        233      43
 
ApoI r'AATT_y

Cuts at:      0     132     276 
   Size:        132     144
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     221     276 
   Size:        221      55
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      12     276 
   Size:         12     264
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22     247     276 
   Size:         22     225      29

  Fragments arranged by size: 

                 225      29      22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      21     276 
   Size:         21     255
 
BsiEI CG_ry'CG

Cuts at:      0      92     276 
   Size:         92     184
 
BsiHKAI G_wGCw'C

Cuts at:      0     172     276 
   Size:        172     104
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     148     276 
   Size:        148     128
 
Bsp1286I G_dGCh'C

Cuts at:      0     172     276 
   Size:        172     104
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     201     276 
   Size:        201      75
 
BsrFI r'CCGG_y

Cuts at:      0     206     276 
   Size:        206      70
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      73     167     276 
   Size:         73      94     109

  Fragments arranged by size: 

                 109      94      73
 
BtgI C'CryG_G

Cuts at:      0      41     276 
   Size:         41     235
 
BtsI GCAGTG_nn'

Cuts at:      0      11     276 
   Size:         11     265
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     276 
   Size:         87     189
 
NaeI GCC'GGC

Cuts at:      0     208     276 
   Size:        208      68
 
NcoI C'CATG_G

Cuts at:      0      41     276 
   Size:         41     235
 
NgoMIV G'CCGG_C

Cuts at:      0     206     276 
   Size:        206      70
 
StyI C'CwwG_G

Cuts at:      0      41     276 
   Size:         41     235
 

 Enzymes that do cut:

     AcuI     AlfI     ApoI     BmrI     BsaI    BseRI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrFI   BstAPI     BtgI     BtsI
  Eco57MI     NaeI     NcoI   NgoMIV     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiWI
     BsmI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NarI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI