(Linear) (Six-base) MAPSORT of: KIBB6728.seq  Check: 6817  from: 1  to: 411



KIBB6728.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

BanII G_rGCy'C

Cuts at:      0      86     409     411 
   Size:         86     323       2

  Fragments arranged by size: 

                 323      86       2
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     159     193     411 
   Size:        159      34     218

  Fragments arranged by size: 

                 218     159      34
 
BclI T'GATC_A

Cuts at:      0      50     411 
   Size:         50     361
 
BlpI GC'TnA_GC

Cuts at:      0     268     411 
   Size:        268     143
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      24     411 
   Size:         24     387
 
BsiHKAI G_wGCw'C

Cuts at:      0     409     411 
   Size:        409       2
 
BsmI GAATG_Cn'

Cuts at:      0     258     411 
   Size:        258     153
 
Bsp1286I G_dGCh'C

Cuts at:      0      86     409     411 
   Size:         86     323       2

  Fragments arranged by size: 

                 323      86       2
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     323     411 
   Size:        323      88
 
BsrDI GCAATG_nn'

Cuts at:      0      22     411 
   Size:         22     389
 
BsrFI r'CCGG_y

Cuts at:      0     156     411 
   Size:        156     255
 
BstYI r'GATC_y

Cuts at:      0     199     411 
   Size:        199     212
 
EaeI y'GGCC_r

Cuts at:      0     204     411 
   Size:        204     207
 
EarI CTCTTCn'nnn_

Cuts at:      0     373     411 
   Size:        373      38
 
EcoICRI GAG'CTC

Cuts at:      0     407     411 
   Size:        407       4
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      24     411 
   Size:         24     387
 
FspI TGC'GCA

Cuts at:      0       8     319     355     411 
   Size:          8     311      36      56

  Fragments arranged by size: 

                 311      56      36       8
 
HaeII r_GCGC'y

Cuts at:      0     347     411 
   Size:        347      64
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     272     304     411 
   Size:        272      32     107

  Fragments arranged by size: 

                 272     107      32
 
MspA1I CmG'CkG

Cuts at:      0     210     411 
   Size:        210     201
 
NaeI GCC'GGC

Cuts at:      0     158     411 
   Size:        158     253
 
NgoMIV G'CCGG_C

Cuts at:      0     156     411 
   Size:        156     255
 
SacI G_AGCT'C

Cuts at:      0     409     411 
   Size:        409       2
 
SfcI C'TryA_G

Cuts at:      0      72     411 
   Size:         72     339
 

 Enzymes that do cut:

    BanII     BcgI     BclI     BlpI     BpmI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrDI    BsrFI    BstYI     EaeI     EarI  EcoICRI  Eco57MI
     FspI    HaeII    Hin4I   MspA1I     NaeI   NgoMIV     SacI     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI     BbsI    BbvCI    BciVI    BfrBI     BglI
    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI