(Linear) (Six-base) MAPSORT of: KIBB6729.seq  Check: 7204  from: 1  to: 276



KIBB6729.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     276 
   Size:         48     228
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     229     276 
   Size:        229      47
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49      64     276 
   Size:         49      15     212

  Fragments arranged by size: 

                 212      49      15
 
BtgI C'CryG_G

Cuts at:      0       9     276 
   Size:          9     267
 
EaeI y'GGCC_r

Cuts at:      0      12     276 
   Size:         12     264
 
EarI CTCTTCn'nnn_

Cuts at:      0     179     276 
   Size:        179      97
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     276 
   Size:         48     228
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     276 
   Size:        205      71
 
MscI TGG'CCA

Cuts at:      0      14     276 
   Size:         14     262
 
SspI AAT'ATT

Cuts at:      0     249     276 
   Size:        249      27
 
XmnI GAAnn'nnTTC

Cuts at:      0     188     276 
   Size:        188      88
 

 Enzymes that do cut:

     AcuI     BmrI     BsgI     BtgI     EaeI     EarI  Eco57MI     MmeI
     MscI     SspI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI