(Linear) (Six-base) MAPSORT of: KIBB6732.seq  Check: 5180  from: 1  to: 390



KIBB6732.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     154     390 
   Size:        154     236
 
AvaI C'yCGr_G

Cuts at:      0     295     390 
   Size:        295      95
 
BanI G'GyrC_C

Cuts at:      0     187     334     390 
   Size:        187     147      56

  Fragments arranged by size: 

                 187     147      56
 
BanII G_rGCy'C

Cuts at:      0      94     390 
   Size:         94     296
 
BbeI G_GCGC'C

Cuts at:      0     191     390 
   Size:        191     199
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     297     331     390 
   Size:        297      34      59

  Fragments arranged by size: 

                 297      59      34
 
Bme1580I G_kGCm'C

Cuts at:      0     263     339     390 
   Size:        263      76      51

  Fragments arranged by size: 

                 263      76      51
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     166     390 
   Size:        166     224
 
BsaHI Gr'CG_yC

Cuts at:      0     188     390 
   Size:        188     202
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      88     118     390 
   Size:         88      30     272

  Fragments arranged by size: 

                 272      88      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     158     390 
   Size:        158     232
 
BseYI C'CCAG_C

Cuts at:      0     174     390 
   Size:        174     216
 
BsiHKAI G_wGCw'C

Cuts at:      0      85      94     390 
   Size:         85       9     296

  Fragments arranged by size: 

                 296      85       9
 
Bsp1286I G_dGCh'C

Cuts at:      0      85      94     263     339     390 
   Size:         85       9     169      76      51

  Fragments arranged by size: 

                 169      85      76      51       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      44      58     390 
   Size:         44      14     332

  Fragments arranged by size: 

                 332      44      14
 
BsrFI r'CCGG_y

Cuts at:      0     184     190     390 
   Size:        184       6     200

  Fragments arranged by size: 

                 200     184       6
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     210     390 
   Size:        210     180
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     180     390 
   Size:        180     210
 
BtsI GCAGTG_nn'

Cuts at:      0      60     390 
   Size:         60     330
 
EaeI y'GGCC_r

Cuts at:      0     134     200     278     390 
   Size:        134      66      78     112

  Fragments arranged by size: 

                 134     112      78      66
 
EarI CTCTTCn'nnn_

Cuts at:      0      49     390 
   Size:         49     341
 
EcoICRI GAG'CTC

Cuts at:      0      92     390 
   Size:         92     298
 
EcoO109I rG'GnC_Cy

Cuts at:      0     120     390 
   Size:        120     270
 
HaeII r_GCGC'y

Cuts at:      0     191     390 
   Size:        191     199
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      88     120     140     172     390 
   Size:         88      32      20      32     218

  Fragments arranged by size: 

                 218      88      32      32      20
 
KasI G'GCGC_C

Cuts at:      0     187     390 
   Size:        187     203
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     120     390 
   Size:        120     270
 
MscI TGG'CCA

Cuts at:      0     280     390 
   Size:        280     110
 
MspA1I CmG'CkG

Cuts at:      0     101     390 
   Size:        101     289
 
NaeI GCC'GGC

Cuts at:      0     192     390 
   Size:        192     198
 
NarI GG'CG_CC

Cuts at:      0     188     390 
   Size:        188     202
 
NgoMIV G'CCGG_C

Cuts at:      0     190     390 
   Size:        190     200
 
PstI C_TGCA'G

Cuts at:      0     290     390 
   Size:        290     100
 
SacI G_AGCT'C

Cuts at:      0      94     390 
   Size:         94     296
 
SapI GCTCTTCn'nnn_

Cuts at:      0      49     390 
   Size:         49     341
 
SfcI C'TryA_G

Cuts at:      0     286     390 
   Size:        286     104
 
SfoI GGC'GCC

Cuts at:      0     189     390 
   Size:        189     201
 
SgrAI Cr'CCGG_yG

Cuts at:      0     184     390 
   Size:        184     206
 
SspI AAT'ATT

Cuts at:      0     330     390 
   Size:        330      60
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     370     390 
   Size:        370      20
 

 Enzymes that do cut:

     AhdI     AvaI     BanI    BanII     BbeI     BcgI Bme1580I     BplI
    BsaHI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BspMI    BsrFI
    BstXI    BtgZI     BtsI     EaeI     EarI  EcoICRI EcoO109I    HaeII
    Hin4I     KasI     MmeI     MscI   MspA1I     NaeI     NarI   NgoMIV
     PstI     SacI     SapI     SfcI     SfoI    SgrAI     SspI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MslI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI