(Linear) (Six-base) MAPSORT of: KIBB6734.seq  Check: 3409  from: 1  to: 414



KIBB6734.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     295     414 
   Size:        295     119
 
AccI GT'mk_AC

Cuts at:      0     344     414 
   Size:        344      70
 
ApaI G_GGCC'C

Cuts at:      0     302     414 
   Size:        302     112
 
AvaI C'yCGr_G

Cuts at:      0     295     414 
   Size:        295     119
 
BanI G'GyrC_C

Cuts at:      0     175     414 
   Size:        175     239
 
BanII G_rGCy'C

Cuts at:      0     296     302     414 
   Size:        296       6     112

  Fragments arranged by size: 

                 296     112       6
 
Bme1580I G_kGCm'C

Cuts at:      0     302     414 
   Size:        302     112
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     414 
   Size:        241     173
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     414 
   Size:        337      77
 
BsaWI w'CCGG_w

Cuts at:      0     332     414 
   Size:        332      82
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       1     238     414 
   Size:          1     237     176

  Fragments arranged by size: 

                 237     176       1
 
BseYI C'CCAG_C

Cuts at:      0     146     414 
   Size:        146     268
 
BsiEI CG_ry'CG

Cuts at:      0     156     414 
   Size:        156     258
 
Bsp1286I G_dGCh'C

Cuts at:      0     296     302     414 
   Size:        296       6     112

  Fragments arranged by size: 

                 296     112       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     295     414 
   Size:        295     119
 
BstEII G'GTnAC_C

Cuts at:      0     282     414 
   Size:        282     132
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      23     211     414 
   Size:         23     188     203

  Fragments arranged by size: 

                 203     188      23
 
BtsI GCAGTG_nn'

Cuts at:      0     204     414 
   Size:        204     210
 
EaeI y'GGCC_r

Cuts at:      0     153     414 
   Size:        153     261
 
EagI C'GGCC_G

Cuts at:      0     153     414 
   Size:        153     261
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     414 
   Size:        241     173
 
EcoO109I rG'GnC_Cy

Cuts at:      0     299     414 
   Size:        299     115
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      61      93     414 
   Size:         61      32     321

  Fragments arranged by size: 

                 321      61      32
 
HindIII A'AGCT_T

Cuts at:      0     337     414 
   Size:        337      77
 
MslI CAynn'nnrTG

Cuts at:      0     209     414 
   Size:        209     205
 
PspOMI G'GGCC_C

Cuts at:      0     298     414 
   Size:        298     116
 
SmaI CCC'GGG

Cuts at:      0     297     414 
   Size:        297     117
 
SmlI C'TyrA_G

Cuts at:      0     352     414 
   Size:        352      62
 
SrfI GCCC'GGGC

Cuts at:      0     297     414 
   Size:        297     117
 
StuI AGG'CCT

Cuts at:      0       8     414 
   Size:          8     406
 
StyI C'CwwG_G

Cuts at:      0     167     414 
   Size:        167     247
 
XmaI C'CCGG_G

Cuts at:      0     295     414 
   Size:        295     119
 

 Enzymes that do cut:

     AarI     AccI     ApaI     AvaI     BanI    BanII Bme1580I     BpmI
    BpuEI    BsaWI    BseRI    BseYI    BsiEI Bsp1286I    BspMI   BstEII
    BstXI     BtsI     EaeI     EagI  Eco57MI EcoO109I    Hin4I  HindIII
     MslI   PspOMI     SmaI     SmlI     SrfI     StuI     StyI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SspI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmnI     ZraI