(Linear) (Six-base) MAPSORT of: KIBB6739.seq  Check: 9758  from: 1  to: 294



KIBB6739.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AflIII A'CryG_T

Cuts at:      0     174     294 
   Size:        174     120
 
ApoI r'AATT_y

Cuts at:      0     249     294 
   Size:        249      45
 
BglII A'GATC_T

Cuts at:      0     286     294 
   Size:        286       8
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      85     294 
   Size:         85     209
 
BsaAI yAC'GTr

Cuts at:      0     175     294 
   Size:        175     119
 
BsmI GAATG_Cn'

Cuts at:      0      53     294 
   Size:         53     241
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     100     294 
   Size:        100     194
 
BsrDI GCAATG_nn'

Cuts at:      0     185     294 
   Size:        185     109
 
BstYI r'GATC_y

Cuts at:      0     286     294 
   Size:        286       8
 
EarI CTCTTCn'nnn_

Cuts at:      0     208     294 
   Size:        208      86
 
PmlI CAC'GTG

Cuts at:      0     175     294 
   Size:        175     119
 
StyI C'CwwG_G

Cuts at:      0     124     294 
   Size:        124     170
 
XbaI T'CTAG_A

Cuts at:      0      73     294 
   Size:         73     221
 
XmnI GAAnn'nnTTC

Cuts at:      0     149     294 
   Size:        149     145
 

 Enzymes that do cut:

   AflIII     ApoI    BglII     BplI    BsaAI     BsmI    BspMI    BsrDI
    BstYI     EarI     PmlI     StyI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XcmI     XhoI     XmaI     ZraI