(Linear) (Six-base) MAPSORT of: KIBB6740.seq  Check: 2779  from: 1  to: 426



KIBB6740.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      27      29     426 
   Size:         27       2     397

  Fragments arranged by size: 

                 397      27       2
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     237     426 
   Size:        237     189
 
ApaI G_GGCC'C

Cuts at:      0     369     426 
   Size:        369      57
 
ApoI r'AATT_y

Cuts at:      0     159     426 
   Size:        159     267
 
BamHI G'GATC_C

Cuts at:      0     102     426 
   Size:        102     324
 
BanII G_rGCy'C

Cuts at:      0     369     426 
   Size:        369      57
 
Bme1580I G_kGCm'C

Cuts at:      0     369     426 
   Size:        369      57
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     108     318     426 
   Size:        108     210     108

  Fragments arranged by size: 

                 210     108     108
 
BsaAI yAC'GTr

Cuts at:      0     110     426 
   Size:        110     316
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      76     426 
   Size:         76     350
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       7     426 
   Size:          7     419
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     343     426 
   Size:        343      83
 
Bsp1286I G_dGCh'C

Cuts at:      0     369     426 
   Size:        369      57
 
BspHI T'CATG_A

Cuts at:      0     269     426 
   Size:        269     157
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      27      29     426 
   Size:         27       2     397

  Fragments arranged by size: 

                 397      27       2
 
BsrGI T'GTAC_A

Cuts at:      0     134     426 
   Size:        134     292
 
BstYI r'GATC_y

Cuts at:      0     102     426 
   Size:        102     324
 
BtgI C'CryG_G

Cuts at:      0     314     360     378     426 
   Size:        314      46      18      48

  Fragments arranged by size: 

                 314      48      46      18
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     126     426 
   Size:        126     300
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      73     426 
   Size:         73     353
 
EaeI y'GGCC_r

Cuts at:      0      90     426 
   Size:         90     336
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     237     426 
   Size:        237     189
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      58     426 
   Size:         58     368
 
EcoO109I rG'GnC_Cy

Cuts at:      0     129     365     426 
   Size:        129     236      61

  Fragments arranged by size: 

                 236     129      61
 
MslI CAynn'nnrTG

Cuts at:      0     118     426 
   Size:        118     308
 
MspA1I CmG'CkG

Cuts at:      0     362     380     426 
   Size:        362      18      46

  Fragments arranged by size: 

                 362      46      18
 
NcoI C'CATG_G

Cuts at:      0     314     426 
   Size:        314     112
 
NspI r_CATG'y

Cuts at:      0      12     426 
   Size:         12     414
 
PpuMI rG'GwC_Cy

Cuts at:      0     129     426 
   Size:        129     297
 
PspOMI G'GGCC_C

Cuts at:      0     365     426 
   Size:        365      61
 
SacII CC_GC'GG

Cuts at:      0     363     381     426 
   Size:        363      18      45

  Fragments arranged by size: 

                 363      45      18
 
StyI C'CwwG_G

Cuts at:      0     314     398     426 
   Size:        314      84      28

  Fragments arranged by size: 

                 314      84      28
 
TatI w'GTAC_w

Cuts at:      0     134     235     426 
   Size:        134     101     191

  Fragments arranged by size: 

                 191     134     101
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      94     126     426 
   Size:         94      32     300

  Fragments arranged by size: 

                 300      94      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     127     426 
   Size:        127     299
 

 Enzymes that do cut:

     AarI     AcuI     ApaI     ApoI    BamHI    BanII Bme1580I     BmrI
    BsaAI    BseRI     BsgI    BsmBI Bsp1286I    BspHI    BspMI    BsrGI
    BstYI     BtgI    BtgZI     DrdI     EaeI  Eco57MI    EcoNI EcoO109I
     MslI   MspA1I     NcoI     NspI    PpuMI   PspOMI    SacII     StyI
     TatI     TstI  Tth111I

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII
     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI