(Linear) (Six-base) MAPSORT of: KIBB6741.seq  Check: 1164  from: 1  to: 294



KIBB6741.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     158     294 
   Size:        158     136
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     177     294 
   Size:        163      14     117

  Fragments arranged by size: 

                 163     117      14
 
AlwNI CAG_nnn'CTG

Cuts at:      0     141     294 
   Size:        141     153
 
BclI T'GATC_A

Cuts at:      0     155     294 
   Size:        155     139
 
BglII A'GATC_T

Cuts at:      0     125     215     294 
   Size:        125      90      79

  Fragments arranged by size: 

                 125      90      79
 
BlpI GC'TnA_GC

Cuts at:      0      68     294 
   Size:         68     226
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     214     294 
   Size:        214      80
 
BspHI T'CATG_A

Cuts at:      0      35     294 
   Size:         35     259
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     158     294 
   Size:        158     136
 
BstYI r'GATC_y

Cuts at:      0     125     215     294 
   Size:        125      90      79

  Fragments arranged by size: 

                 125      90      79
 
BtsI GCAGTG_nn'

Cuts at:      0      20     294 
   Size:         20     274
 
EarI CTCTTCn'nnn_

Cuts at:      0     139     294 
   Size:        139     155
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     177     294 
   Size:        163      14     117

  Fragments arranged by size: 

                 163     117      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     135     294 
   Size:        135     159
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     147     294 
   Size:        147     147
 
PasI CC'CwG_GG

Cuts at:      0      57     294 
   Size:         57     237
 
PstI C_TGCA'G

Cuts at:      0      15     294 
   Size:         15     279
 
SapI GCTCTTCn'nnn_

Cuts at:      0     139     294 
   Size:        139     155
 
SfcI C'TryA_G

Cuts at:      0      11     294 
   Size:         11     283
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      71     103     294 
   Size:         71      32     191

  Fragments arranged by size: 

                 191      71      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     236     294 
   Size:        236      58
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     BclI    BglII     BlpI     BmrI    BspHI
    BspMI    BstYI     BtsI     EarI  Eco57MI    EcoNI     FalI     PasI
     PstI     SapI     SfcI     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI Bme1580I
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI