(Linear) (Six-base) MAPSORT of: KIBB6745.seq  Check: 7767  from: 1  to: 303



KIBB6745.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     119     247     303 
   Size:        113       6     128      56

  Fragments arranged by size: 

                 128     113      56       6
 
AfeI AGC'GCT

Cuts at:      0      90     303 
   Size:         90     213
 
AlwNI CAG_nnn'CTG

Cuts at:      0     123     303 
   Size:        123     180
 
ApoI r'AATT_y

Cuts at:      0     242     303 
   Size:        242      61
 
AvaI C'yCGr_G

Cuts at:      0      34     303 
   Size:         34     269
 
BanII G_rGCy'C

Cuts at:      0       7     303 
   Size:          7     296
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     139     303 
   Size:        139     164
 
BclI T'GATC_A

Cuts at:      0     179     303 
   Size:        179     124
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     303 
   Size:        193     110
 
BsiHKAI G_wGCw'C

Cuts at:      0       7     303 
   Size:          7     296
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     303 
   Size:          7     296
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     122     263     303 
   Size:        122     141      40

  Fragments arranged by size: 

                 141     122      40
 
BsrGI T'GTAC_A

Cuts at:      0     269     303 
   Size:        269      34
 
BtsI GCAGTG_nn'

Cuts at:      0     124     195     203     303 
   Size:        124      71       8     100

  Fragments arranged by size: 

                 124     100      71       8
 
EcoICRI GAG'CTC

Cuts at:      0       5     303 
   Size:          5     298
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     119     193     247     303 
   Size:        113       6      74      54      56

  Fragments arranged by size: 

                 113      74      56      54       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     111     303 
   Size:        111     192
 
HaeII r_GCGC'y

Cuts at:      0      92     303 
   Size:         92     211
 
MspA1I CmG'CkG

Cuts at:      0     267     303 
   Size:        267      36
 
PpuMI rG'GwC_Cy

Cuts at:      0     111     303 
   Size:        111     192
 
PstI C_TGCA'G

Cuts at:      0     119     303 
   Size:        119     184
 
SacI G_AGCT'C

Cuts at:      0       7     303 
   Size:          7     296
 
SfcI C'TryA_G

Cuts at:      0     115     303 
   Size:        115     188
 
SmaI CCC'GGG

Cuts at:      0      36     303 
   Size:         36     267
 
TatI w'GTAC_w

Cuts at:      0     269     303 
   Size:        269      34
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     103     135     303 
   Size:        103      32     168

  Fragments arranged by size: 

                 168     103      32
 
XmaI C'CCGG_G

Cuts at:      0      34     303 
   Size:         34     269
 

 Enzymes that do cut:

     AcuI     AfeI    AlwNI     ApoI     AvaI    BanII     BbsI     BclI
     BpmI  BsiHKAI Bsp1286I    BspMI    BsrGI     BtsI  EcoICRI  Eco57MI
 EcoO109I    HaeII   MspA1I    PpuMI     PstI     SacI     SfcI     SmaI
     TatI     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI