(Linear) (Six-base) MAPSORT of: KIBB6746.seq  Check: 2652  from: 1  to: 432



KIBB6746.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

BbsI GAAGACnn'nnnn_

Cuts at:      0      85     376     432 
   Size:         85     291      56

  Fragments arranged by size: 

                 291      85      56
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     432 
   Size:        205     227
 
BsaAI yAC'GTr

Cuts at:      0     301     432 
   Size:        301     131
 
BsiEI CG_ry'CG

Cuts at:      0      29     432 
   Size:         29     403
 
BsrGI T'GTAC_A

Cuts at:      0     285     432 
   Size:        285     147
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      69     432 
   Size:         69     363
 
DraI TTT'AAA

Cuts at:      0      68     432 
   Size:         68     364
 
EaeI y'GGCC_r

Cuts at:      0      26     432 
   Size:         26     406
 
EagI C'GGCC_G

Cuts at:      0      26     432 
   Size:         26     406
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     227     233     259     265     432 
   Size:        227       6      26       6     167

  Fragments arranged by size: 

                 227     167      26       6       6
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     147     432 
   Size:        147     285
 
PshAI GACnn'nnGTC

Cuts at:      0     380     432 
   Size:        380      52
 
SfcI C'TryA_G

Cuts at:      0     131     137     432 
   Size:        131       6     295

  Fragments arranged by size: 

                 295     131       6
 
SmlI C'TyrA_G

Cuts at:      0     184     432 
   Size:        184     248
 
SwaI ATTT'AAAT

Cuts at:      0      68     432 
   Size:         68     364
 
TatI w'GTAC_w

Cuts at:      0     200     285     432 
   Size:        200      85     147

  Fragments arranged by size: 

                 200     147      85
 
XbaI T'CTAG_A

Cuts at:      0     205     432 
   Size:        205     227
 

 Enzymes that do cut:

     BbsI    BpuEI    BsaAI    BsiEI    BsrGI    BtgZI     DraI     EaeI
     EagI    Hin4I     MmeI    PshAI     SfcI     SmlI     SwaI     TatI
     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
     BtsI     ClaI    CspCI   DraIII     DrdI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI    TaqII     TstI  Tth111I     XcmI     XhoI
     XmaI     XmnI     ZraI