(Linear) (Six-base) MAPSORT of: KIBB6747.seq  Check: 8849  from: 1  to: 306



KIBB6747.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AccI GT'mk_AC

Cuts at:      0      14     306 
   Size:         14     292
 
ApoI r'AATT_y

Cuts at:      0      83     306 
   Size:         83     223
 
BanII G_rGCy'C

Cuts at:      0      50     306 
   Size:         50     256
 
BclI T'GATC_A

Cuts at:      0     138     306 
   Size:        138     168
 
BlpI GC'TnA_GC

Cuts at:      0      67     306 
   Size:         67     239
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     257     287     306 
   Size:        257      30      19

  Fragments arranged by size: 

                 257      30      19
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58     306 
   Size:         58     248
 
Bsp1286I G_dGCh'C

Cuts at:      0      50     306 
   Size:         50     256
 
BstYI r'GATC_y

Cuts at:      0      54     306 
   Size:         54     252
 
DraI TTT'AAA

Cuts at:      0     149     306 
   Size:        149     157
 
EarI CTCTTCn'nnn_

Cuts at:      0     209     306 
   Size:        209      97
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     306 
   Size:         19     287
 
MslI CAynn'nnrTG

Cuts at:      0     134     306 
   Size:        134     172
 
NdeI CA'TA_TG

Cuts at:      0     210     306 
   Size:        210      96
 
NspI r_CATG'y

Cuts at:      0     133     306 
   Size:        133     173
 
SfcI C'TryA_G

Cuts at:      0      38     188     306 
   Size:         38     150     118

  Fragments arranged by size: 

                 150     118      38
 
SspI AAT'ATT

Cuts at:      0     243     306 
   Size:        243      63
 
SwaI ATTT'AAAT

Cuts at:      0     149     306 
   Size:        149     157
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     122     306 
   Size:        122     184
 

 Enzymes that do cut:

     AccI     ApoI    BanII     BclI     BlpI    BsaXI    BseRI Bsp1286I
    BstYI     DraI     EarI     EciI     MslI     NdeI     NspI     SfcI
     SspI     SwaI    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI