(Linear) (Six-base) MAPSORT of: KIBB6747.seq Check: 8849 from: 1 to: 306 KIBB6747.orf With 182 enzymes: SgfI * June 26, 2008 10:48 .. AccI GT'mk_AC Cuts at: 0 14 306 Size: 14 292 ApoI r'AATT_y Cuts at: 0 83 306 Size: 83 223 BanII G_rGCy'C Cuts at: 0 50 306 Size: 50 256 BclI T'GATC_A Cuts at: 0 138 306 Size: 138 168 BlpI GC'TnA_GC Cuts at: 0 67 306 Size: 67 239 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 257 287 306 Size: 257 30 19 Fragments arranged by size: 257 30 19 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 58 306 Size: 58 248 Bsp1286I G_dGCh'C Cuts at: 0 50 306 Size: 50 256 BstYI r'GATC_y Cuts at: 0 54 306 Size: 54 252 DraI TTT'AAA Cuts at: 0 149 306 Size: 149 157 EarI CTCTTCn'nnn_ Cuts at: 0 209 306 Size: 209 97 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 306 Size: 19 287 MslI CAynn'nnrTG Cuts at: 0 134 306 Size: 134 172 NdeI CA'TA_TG Cuts at: 0 210 306 Size: 210 96 NspI r_CATG'y Cuts at: 0 133 306 Size: 133 173 SfcI C'TryA_G Cuts at: 0 38 188 306 Size: 38 150 118 Fragments arranged by size: 150 118 38 SspI AAT'ATT Cuts at: 0 243 306 Size: 243 63 SwaI ATTT'AAAT Cuts at: 0 149 306 Size: 149 157 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 122 306 Size: 122 184 Enzymes that do cut: AccI ApoI BanII BclI BlpI BsaXI BseRI Bsp1286I BstYI DraI EarI EciI MslI NdeI NspI SfcI SspI SwaI TaqII Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI