(Linear) (Six-base) MAPSORT of: KIBB6749.seq  Check: 443  from: 1  to: 309



KIBB6749.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

ApoI r'AATT_y

Cuts at:      0     137     309 
   Size:        137     172
 
BanI G'GyrC_C

Cuts at:      0      43     107     309 
   Size:         43      64     202

  Fragments arranged by size: 

                 202      64      43
 
BbeI G_GCGC'C

Cuts at:      0     111     309 
   Size:        111     198
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     309 
   Size:        208     101
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     256     309 
   Size:        160      96      53

  Fragments arranged by size: 

                 160      96      53
 
BsaHI Gr'CG_yC

Cuts at:      0     108     206     309 
   Size:        108      98     103

  Fragments arranged by size: 

                 108     103      98
 
BsaWI w'CCGG_w

Cuts at:      0     142     309 
   Size:        142     167
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     172     309 
   Size:        172     137
 
BsiHKAI G_wGCw'C

Cuts at:      0     227     309 
   Size:        227      82
 
Bsp1286I G_dGCh'C

Cuts at:      0     227     309 
   Size:        227      82
 
BsrFI r'CCGG_y

Cuts at:      0      96     309 
   Size:         96     213
 
BssSI C'ACGA_G

Cuts at:      0     164     309 
   Size:        164     145
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     258     309 
   Size:        258      51
 
BtgI C'CryG_G

Cuts at:      0     251     309 
   Size:        251      58
 
BtsI GCAGTG_nn'

Cuts at:      0     245     309 
   Size:        245      64
 
EaeI y'GGCC_r

Cuts at:      0      98     309 
   Size:         98     211
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     309 
   Size:        208     101
 
HaeII r_GCGC'y

Cuts at:      0     111     309 
   Size:        111     198
 
KasI G'GCGC_C

Cuts at:      0     107     309 
   Size:        107     202
 
MslI CAynn'nnrTG

Cuts at:      0     250     256     309 
   Size:        250       6      53

  Fragments arranged by size: 

                 250      53       6
 
NarI GG'CG_CC

Cuts at:      0     108     309 
   Size:        108     201
 
NcoI C'CATG_G

Cuts at:      0     251     309 
   Size:        251      58
 
PfoI T'CCnGG_A

Cuts at:      0     200     309 
   Size:        200     109
 
SfoI GGC'GCC

Cuts at:      0     109     309 
   Size:        109     200
 
SmlI C'TyrA_G

Cuts at:      0     175     271     309 
   Size:        175      96      38

  Fragments arranged by size: 

                 175      96      38
 
StyI C'CwwG_G

Cuts at:      0      81     251     309 
   Size:         81     170      58

  Fragments arranged by size: 

                 170      81      58
 

 Enzymes that do cut:

     ApoI     BanI     BbeI     BpmI    BpuEI    BsaHI    BsaWI    BseRI
  BsiHKAI Bsp1286I    BsrFI    BssSI    BstXI     BtgI     BtsI     EaeI
  Eco57MI    HaeII     KasI     MslI     NarI     NcoI     PfoI     SfoI
     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaXI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI