(Linear) (Six-base) MAPSORT of: KIBB6753.seq  Check: 1633  from: 1  to: 315



KIBB6753.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      28     315 
   Size:         28     287
 
ApoI r'AATT_y

Cuts at:      0     235     315 
   Size:        235      80
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      51     315 
   Size:         51     264
 
Bpu10I CC'TnA_GC

Cuts at:      0     118     315 
   Size:        118     197
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     315 
   Size:        271      44
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      85     115     315 
   Size:         85      30     200

  Fragments arranged by size: 

                 200      85      30
 
BspHI T'CATG_A

Cuts at:      0     125     315 
   Size:        125     190
 
BsrBI CCG'CTC

Cuts at:      0      33     315 
   Size:         33     282
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     172     315 
   Size:        172     143
 
EcoO109I rG'GnC_Cy

Cuts at:      0     165     315 
   Size:        165     150
 
HindIII A'AGCT_T

Cuts at:      0     120     315 
   Size:        120     195
 
PpuMI rG'GwC_Cy

Cuts at:      0     165     315 
   Size:        165     150
 
PsiI TTA'TAA

Cuts at:      0      89     315 
   Size:         89     226
 
SfcI C'TryA_G

Cuts at:      0      20     315 
   Size:         20     295
 
SmlI C'TyrA_G

Cuts at:      0     286     315 
   Size:        286      29
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      70     166     315 
   Size:         70      96     149

  Fragments arranged by size: 

                 149      96      70
 

 Enzymes that do cut:

    AlwNI     ApoI     BbsI   Bpu10I    BpuEI    BsaXI    BspHI    BsrBI
     DrdI EcoO109I  HindIII    PpuMI     PsiI     SfcI     SmlI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI