(Linear) (Six-base) MAPSORT of: KIBB6754.seq  Check: 4928  from: 1  to: 438



KIBB6754.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     384     406     438 
   Size:        199     185      22      32

  Fragments arranged by size: 

                 199     185      32      22
 
AlwNI CAG_nnn'CTG

Cuts at:      0     384     438 
   Size:        384      54
 
BamHI G'GATC_C

Cuts at:      0     342     438 
   Size:        342      96
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     390     438 
   Size:        390      48
 
BclI T'GATC_A

Cuts at:      0     134     438 
   Size:        134     304
 
BfrBI ATG'CAT

Cuts at:      0     223     438 
   Size:        223     215
 
Bme1580I G_kGCm'C

Cuts at:      0      38     438 
   Size:         38     400
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     438 
   Size:         85     353
 
BsaAI yAC'GTr

Cuts at:      0      30     438 
   Size:         30     408
 
BsaBI GATnn'nnATC

Cuts at:      0     329     438 
   Size:        329     109
 
BsaWI w'CCGG_w

Cuts at:      0      81     438 
   Size:         81     357
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      58      88     438 
   Size:         58      30     350

  Fragments arranged by size: 

                 350      58      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     224     438 
   Size:        224     214
 
BsiEI CG_ry'CG

Cuts at:      0      82     438 
   Size:         82     356
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     438 
   Size:         38     400
 
BspHI T'CATG_A

Cuts at:      0     131     438 
   Size:        131     307
 
BsrFI r'CCGG_y

Cuts at:      0     251     438 
   Size:        251     187
 
BsrGI T'GTAC_A

Cuts at:      0     153     438 
   Size:        153     285
 
BssSI C'ACGA_G

Cuts at:      0      55     438 
   Size:         55     383
 
BstYI r'GATC_y

Cuts at:      0     342     438 
   Size:        342      96
 
EaeI y'GGCC_r

Cuts at:      0      51     438 
   Size:         51     387
 
EarI CTCTTCn'nnn_

Cuts at:      0     175     438 
   Size:        175     263
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     199     384     406     438 
   Size:         85     114     185      22      32

  Fragments arranged by size: 

                 185     114      85      32      22
 
EcoO109I rG'GnC_Cy

Cuts at:      0     381     438 
   Size:        381      57
 
NaeI GCC'GGC

Cuts at:      0     253     438 
   Size:        253     185
 
NgoMIV G'CCGG_C

Cuts at:      0     251     438 
   Size:        251     187
 
NsiI A_TGCA'T

Cuts at:      0     225     438 
   Size:        225     213
 
PfoI T'CCnGG_A

Cuts at:      0      62     377     438 
   Size:         62     315      61

  Fragments arranged by size: 

                 315      62      61
 
PpuMI rG'GwC_Cy

Cuts at:      0     381     438 
   Size:        381      57
 
StyI C'CwwG_G

Cuts at:      0     338     438 
   Size:        338     100
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     257     438 
   Size:        257     181
 
TatI w'GTAC_w

Cuts at:      0     153     438 
   Size:        153     285
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     373     405     438 
   Size:        373      32      33

  Fragments arranged by size: 

                 373      33      32
 

 Enzymes that do cut:

     AcuI    AlwNI    BamHI     BbsI     BclI    BfrBI Bme1580I     BpmI
    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BsiEI Bsp1286I    BspHI
    BsrFI    BsrGI    BssSI    BstYI     EaeI     EarI  Eco57MI EcoO109I
     NaeI   NgoMIV     NsiI     PfoI    PpuMI     StyI    TaqII     TatI
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaHI    BseYI
     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI
    BsrDI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NarI
     NcoI     NdeI     NheI     NotI     NruI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI