(Linear) (Six-base) MAPSORT of: KIBB6755.seq  Check: 2451  from: 1  to: 315



KIBB6755.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     315 
   Size:        139     176
 
AvaI C'yCGr_G

Cuts at:      0      54     106     315 
   Size:         54      52     209

  Fragments arranged by size: 

                 209      54      52
 
BbvCI CC'TCA_GC

Cuts at:      0     125     315 
   Size:        125     190
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     183     287     315 
   Size:        183     104      28

  Fragments arranged by size: 

                 183     104      28
 
Bpu10I CC'TnA_GC

Cuts at:      0     125     315 
   Size:        125     190
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     109     315 
   Size:        109     206
 
BsaHI Gr'CG_yC

Cuts at:      0      12     315 
   Size:         12     303
 
BsaWI w'CCGG_w

Cuts at:      0     254     315 
   Size:        254      61
 
BspEI T'CCGG_A

Cuts at:      0     254     315 
   Size:        254      61
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      39     315 
   Size:         39     276
 
DraI TTT'AAA

Cuts at:      0     276     315 
   Size:        276      39
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     315 
   Size:        139     176
 
EcoO109I rG'GnC_Cy

Cuts at:      0      51     315 
   Size:         51     264
 
HaeII r_GCGC'y

Cuts at:      0     125     315 
   Size:        125     190
 
PpuMI rG'GwC_Cy

Cuts at:      0      51     315 
   Size:         51     264
 
PstI C_TGCA'G

Cuts at:      0     135     315 
   Size:        135     180
 
SfcI C'TryA_G

Cuts at:      0     116     131     315 
   Size:        116      15     184

  Fragments arranged by size: 

                 184     116      15
 
StyI C'CwwG_G

Cuts at:      0      99     315 
   Size:         99     216
 

 Enzymes that do cut:

     AcuI     AvaI    BbvCI     BmrI   Bpu10I     BsaI    BsaHI    BsaWI
    BspEI    BspMI     DraI  Eco57MI EcoO109I    HaeII    PpuMI     PstI
     SfcI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI    BpuEI
    BsaAI    BsaBI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI