(Linear) (Six-base) MAPSORT of: KIBB6757.seq  Check: 3443  from: 1  to: 318



KIBB6757.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AflII C'TTAA_G

Cuts at:      0     301     318 
   Size:        301      17
 
BamHI G'GATC_C

Cuts at:      0     144     318 
   Size:        144     174
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      88     318 
   Size:         88     230
 
Bpu10I CC'TnA_GC

Cuts at:      0     149     318 
   Size:        149     169
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     265     318 
   Size:        265      53
 
BstYI r'GATC_y

Cuts at:      0     144     264     318 
   Size:        144     120      54

  Fragments arranged by size: 

                 144     120      54
 
BtgI C'CryG_G

Cuts at:      0      11     318 
   Size:         11     307
 
EarI CTCTTCn'nnn_

Cuts at:      0     127     318 
   Size:        127     191
 
EcoO109I rG'GnC_Cy

Cuts at:      0     247     318 
   Size:        247      71
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     173     318 
   Size:         60     113     145

  Fragments arranged by size: 

                 145     113      60
 
MspA1I CmG'CkG

Cuts at:      0      13     318 
   Size:         13     305
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     257     318 
   Size:        257      61
 
PpuMI rG'GwC_Cy

Cuts at:      0     247     318 
   Size:        247      71
 
SacII CC_GC'GG

Cuts at:      0      14     318 
   Size:         14     304
 
SmlI C'TyrA_G

Cuts at:      0     301     318 
   Size:        301      17
 
SspI AAT'ATT

Cuts at:      0     240     318 
   Size:        240      78
 
StuI AGG'CCT

Cuts at:      0     255     318 
   Size:        255      63
 
StyI C'CwwG_G

Cuts at:      0     256     279     318 
   Size:        256      23      39

  Fragments arranged by size: 

                 256      39      23
 

 Enzymes that do cut:

    AflII    BamHI     BbsI   Bpu10I    BseRI    BstYI     BtgI     EarI
 EcoO109I     MmeI   MspA1I    PflMI    PpuMI    SacII     SmlI     SspI
     StuI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI