(Linear) (Six-base) MAPSORT of: KIBB6758.seq  Check: 8801  from: 1  to: 441



KIBB6758.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     441 
   Size:         76     365
 
BanI G'GyrC_C

Cuts at:      0     403     441 
   Size:        403      38
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      37     441 
   Size:         37     404
 
BbvCI CC'TCA_GC

Cuts at:      0     375     441 
   Size:        375      66
 
BlpI GC'TnA_GC

Cuts at:      0     115     441 
   Size:        115     326
 
Bme1580I G_kGCm'C

Cuts at:      0     408     441 
   Size:        408      33
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     197     441 
   Size:        197     244
 
Bpu10I CC'TnA_GC

Cuts at:      0     375     441 
   Size:        375      66
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     195     225     400     430     441 
   Size:        195      30     175      30      11

  Fragments arranged by size: 

                 195     175      30      30      11
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22     441 
   Size:         22     419
 
BseYI C'CCAG_C

Cuts at:      0     391     441 
   Size:        391      50
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     441 
   Size:        106     335
 
BsmI GAATG_Cn'

Cuts at:      0     314     441 
   Size:        314     127
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     271     441 
   Size:        271     170
 
Bsp1286I G_dGCh'C

Cuts at:      0     408     441 
   Size:        408      33
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     344     441 
   Size:        344      97
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     391     441 
   Size:        391      50
 
BtgI C'CryG_G

Cuts at:      0      80     441 
   Size:         80     361
 
BtsI GCAGTG_nn'

Cuts at:      0     319     441 
   Size:        319     122
 
EaeI y'GGCC_r

Cuts at:      0      62     441 
   Size:         62     379
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     197     441 
   Size:         76     121     244

  Fragments arranged by size: 

                 244     121      76
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     268     270     300     302     441 
   Size:        268       2      30       2     139

  Fragments arranged by size: 

                 268     139      30       2       2
 
MspA1I CmG'CkG

Cuts at:      0     114     441 
   Size:        114     327
 
SfcI C'TryA_G

Cuts at:      0     398     441 
   Size:        398      43
 
StuI AGG'CCT

Cuts at:      0     133     441 
   Size:        133     308
 
StyI C'CwwG_G

Cuts at:      0      17     441 
   Size:         17     424
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     387     400     419     432     441 
   Size:        387      13      19      13       9

  Fragments arranged by size: 

                 387      19      13      13       9
 
Tth111I GACn'n_nGTC

Cuts at:      0     282     441 
   Size:        282     159
 

 Enzymes that do cut:

     AcuI     BanI     BbsI    BbvCI     BlpI Bme1580I     BpmI   Bpu10I
    BsaXI    BseRI    BseYI     BsgI     BsmI    BsmBI Bsp1286I   BstAPI
    BstXI     BtgI     BtsI     EaeI  Eco57MI    Hin4I   MspA1I     SfcI
     StuI     StyI     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsiEI  BsiHKAI    BsiWI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII     TatI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI