(Linear) (Six-base) MAPSORT of: KIBB6761.seq  Check: 716  from: 1  to: 327



KIBB6761.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

AleI CACnn'nnGTG

Cuts at:      0      51     270     327 
   Size:         51     219      57

  Fragments arranged by size: 

                 219      57      51
 
AlwNI CAG_nnn'CTG

Cuts at:      0      73     171     327 
   Size:         73      98     156

  Fragments arranged by size: 

                 156      98      73
 
AseI AT'TA_AT

Cuts at:      0     113     327 
   Size:        113     214
 
AvaI C'yCGr_G

Cuts at:      0     209     327 
   Size:        209     118
 
BglII A'GATC_T

Cuts at:      0     248     327 
   Size:        248      79
 
BlpI GC'TnA_GC

Cuts at:      0      98     327 
   Size:         98     229
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     327 
   Size:        193     134
 
Bpu10I CC'TnA_GC

Cuts at:      0     161     327 
   Size:        161     166
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     300     327 
   Size:        300      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     136     327 
   Size:        136     191
 
BstYI r'GATC_y

Cuts at:      0     248     327 
   Size:        248      79
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     327 
   Size:        193     134
 
HindIII A'AGCT_T

Cuts at:      0     322     327 
   Size:        322       5
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     327 
   Size:         50     277
 
MslI CAynn'nnrTG

Cuts at:      0      51     270     327 
   Size:         51     219      57

  Fragments arranged by size: 

                 219      57      51
 
MspA1I CmG'CkG

Cuts at:      0      73     327 
   Size:         73     254
 
PasI CC'CwG_GG

Cuts at:      0      87     327 
   Size:         87     240
 
PfoI T'CCnGG_A

Cuts at:      0     278     327 
   Size:        278      49
 
PstI C_TGCA'G

Cuts at:      0      45     327 
   Size:         45     282
 
PvuII CAG'CTG

Cuts at:      0      73     327 
   Size:         73     254
 
SfcI C'TryA_G

Cuts at:      0      41     135     180     327 
   Size:         41      94      45     147

  Fragments arranged by size: 

                 147      94      45      41
 
SmaI CCC'GGG

Cuts at:      0     211     327 
   Size:        211     116
 
XmaI C'CCGG_G

Cuts at:      0     209     327 
   Size:        209     118
 

 Enzymes that do cut:

     AleI    AlwNI     AseI     AvaI    BglII     BlpI     BpmI   Bpu10I
    BsaXI    BspMI    BstYI  Eco57MI  HindIII     MmeI     MslI   MspA1I
     PasI     PfoI     PstI    PvuII     SfcI     SmaI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI Bme1580I
    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI