(Linear) (Six-base) MAPSORT of: KIBB6765.seq  Check: 6711  from: 1  to: 342



KIBB6765.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     189     342 
   Size:        189     153
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     342 
   Size:        271      71
 
AfeI AGC'GCT

Cuts at:      0     108     342 
   Size:        108     234
 
AflIII A'CryG_T

Cuts at:      0     156     342 
   Size:        156     186
 
ApaI G_GGCC'C

Cuts at:      0      98     342 
   Size:         98     244
 
ApoI r'AATT_y

Cuts at:      0     314     342 
   Size:        314      28
 
AvaI C'yCGr_G

Cuts at:      0      33     342 
   Size:         33     309
 
BanII G_rGCy'C

Cuts at:      0      49      98     342 
   Size:         49      49     244

  Fragments arranged by size: 

                 244      49      49
 
BglI GCCn_nnn'nGGC

Cuts at:      0      93     342 
   Size:         93     249
 
Bme1580I G_kGCm'C

Cuts at:      0      98     342 
   Size:         98     244
 
BsaHI Gr'CG_yC

Cuts at:      0      85     342 
   Size:         85     257
 
BsiHKAI G_wGCw'C

Cuts at:      0     226     342 
   Size:        226     116
 
Bsp1286I G_dGCh'C

Cuts at:      0      49      98     226     342 
   Size:         49      49     128     116

  Fragments arranged by size: 

                 128     116      49      49
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     189     342 
   Size:        189     153
 
BssHII G'CGCG_C

Cuts at:      0      56     342 
   Size:         56     286
 
BtsI GCAGTG_nn'

Cuts at:      0     226     342 
   Size:        226     116
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     342 
   Size:        271      71
 
EcoO109I rG'GnC_Cy

Cuts at:      0      37      94      95     342 
   Size:         37      57       1     247

  Fragments arranged by size: 

                 247      57      37       1
 
HaeII r_GCGC'y

Cuts at:      0     110     342 
   Size:        110     232
 
MluI A'CGCG_T

Cuts at:      0     156     342 
   Size:        156     186
 
PpuMI rG'GwC_Cy

Cuts at:      0      37     342 
   Size:         37     305
 
PspOMI G'GGCC_C

Cuts at:      0      94     342 
   Size:         94     248
 
PstI C_TGCA'G

Cuts at:      0     200     342 
   Size:        200     142
 
SfcI C'TryA_G

Cuts at:      0     196     342 
   Size:        196     146
 
SmaI CCC'GGG

Cuts at:      0      35     342 
   Size:         35     307
 
StyI C'CwwG_G

Cuts at:      0     227     342 
   Size:        227     115
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     234     342 
   Size:        234     108
 
XmaI C'CCGG_G

Cuts at:      0      33     342 
   Size:         33     309
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     ApaI     ApoI     AvaI    BanII
     BglI Bme1580I    BsaHI  BsiHKAI Bsp1286I    BspMI   BssHII     BtsI
  Eco57MI EcoO109I    HaeII     MluI    PpuMI   PspOMI     PstI     SfcI
     SmaI     StyI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI