Restriction map for
pF1KB6766
(Linear) (Six-base) MAPSORT of: KIBB6766.seq Check: 9044 from: 1 to: 444
KIBB6766.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 0 387 444
Size: 387 57
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 444
Size: 220 224
AlwNI CAG_nnn'CTG
Cuts at: 0 210 444
Size: 210 234
ApoI r'AATT_y
Cuts at: 0 246 444
Size: 246 198
AvaI C'yCGr_G
Cuts at: 0 56 444
Size: 56 388
BanI G'GyrC_C
Cuts at: 0 51 444
Size: 51 393
BanII G_rGCy'C
Cuts at: 0 17 444
Size: 17 427
BbeI G_GCGC'C
Cuts at: 0 55 444
Size: 55 389
BbsI GAAGACnn'nnnn_
Cuts at: 0 164 444
Size: 164 280
BglI GCCn_nnn'nGGC
Cuts at: 0 60 444
Size: 60 384
Bme1580I G_kGCm'C
Cuts at: 0 143 444
Size: 143 301
BmrI ACTGGGnnnn_n'
Cuts at: 0 138 444
Size: 138 306
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 444
Size: 383 61
BsaI GGTCTCn'nnnn_
Cuts at: 0 262 444
Size: 262 182
BsaHI Gr'CG_yC
Cuts at: 0 52 444
Size: 52 392
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 80 110 444
Size: 80 30 334
Fragments arranged by size:
334 80 30
BseYI C'CCAG_C
Cuts at: 0 252 428 444
Size: 252 176 16
Fragments arranged by size:
252 176 16
BsiEI CG_ry'CG
Cuts at: 0 9 444
Size: 9 435
BsiHKAI G_wGCw'C
Cuts at: 0 17 163 444
Size: 17 146 281
Fragments arranged by size:
281 146 17
Bsp1286I G_dGCh'C
Cuts at: 0 17 143 163 444
Size: 17 126 20 281
Fragments arranged by size:
281 126 20 17
BsrBI CCG'CTC
Cuts at: 0 67 444
Size: 67 377
BstXI CCAn_nnnn'nTGG
Cuts at: 0 309 444
Size: 309 135
EaeI y'GGCC_r
Cuts at: 0 6 444
Size: 6 438
EagI C'GGCC_G
Cuts at: 0 6 444
Size: 6 438
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 101 444
Size: 101 343
EcoICRI GAG'CTC
Cuts at: 0 15 444
Size: 15 429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 383 444
Size: 220 163 61
Fragments arranged by size:
220 163 61
EcoO109I rG'GnC_Cy
Cuts at: 0 306 444
Size: 306 138
HaeII r_GCGC'y
Cuts at: 0 37 55 444
Size: 37 18 389
Fragments arranged by size:
389 37 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 206 238 444
Size: 206 32 206
Fragments arranged by size:
206 206 32
KasI G'GCGC_C
Cuts at: 0 51 444
Size: 51 393
MspA1I CmG'CkG
Cuts at: 0 21 75 210 432 444
Size: 21 54 135 222 12
Fragments arranged by size:
222 135 54 21 12
NarI GG'CG_CC
Cuts at: 0 52 444
Size: 52 392
PasI CC'CwG_GG
Cuts at: 0 309 444
Size: 309 135
PvuII CAG'CTG
Cuts at: 0 210 432 444
Size: 210 222 12
Fragments arranged by size:
222 210 12
SacI G_AGCT'C
Cuts at: 0 17 444
Size: 17 427
SfoI GGC'GCC
Cuts at: 0 53 444
Size: 53 391
SmaI CCC'GGG
Cuts at: 0 58 444
Size: 58 386
StyI C'CwwG_G
Cuts at: 0 302 444
Size: 302 142
TatI w'GTAC_w
Cuts at: 0 218 444
Size: 218 226
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 80 112 444
Size: 80 32 332
Fragments arranged by size:
332 80 32
XmaI C'CCGG_G
Cuts at: 0 56 444
Size: 56 388
Enzymes that do cut:
AccI AcuI AlwNI ApoI AvaI BanI BanII BbeI
BbsI BglI Bme1580I BmrI BpmI BsaI BsaHI BsaXI
BseYI BsiEI BsiHKAI Bsp1286I BsrBI BstXI EaeI EagI
EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MspA1I
NarI PasI PvuII SacI SfoI SmaI StyI TatI
TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI
BsaAI BsaBI BsaWI BseRI BsgI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EarI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI
XmnI ZraI