(Linear) (Six-base) MAPSORT of: KIBB6768.seq  Check: 4086  from: 1  to: 444



KIBB6768.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AleI CACnn'nnGTG

Cuts at:      0     175     444 
   Size:        175     269
 
ApoI r'AATT_y

Cuts at:      0     250     444 
   Size:        250     194
 
AvaI C'yCGr_G

Cuts at:      0      62     105     444 
   Size:         62      43     339

  Fragments arranged by size: 

                 339      62      43
 
BanI G'GyrC_C

Cuts at:      0     169     444 
   Size:        169     275
 
BanII G_rGCy'C

Cuts at:      0     166     444 
   Size:        166     278
 
BclI T'GATC_A

Cuts at:      0      89     444 
   Size:         89     355
 
BmtI G_CTAG'C

Cuts at:      0      19     444 
   Size:         19     425
 
Bpu10I CC'TnA_GC

Cuts at:      0     165     444 
   Size:        165     279
 
BsiEI CG_ry'CG

Cuts at:      0     363     444 
   Size:        363      81
 
Bsp1286I G_dGCh'C

Cuts at:      0     166     444 
   Size:        166     278
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     237     444 
   Size:        237     207
 
BtgI C'CryG_G

Cuts at:      0     176     444 
   Size:        176     268
 
EaeI y'GGCC_r

Cuts at:      0      35     444 
   Size:         35     409
 
MslI CAynn'nnrTG

Cuts at:      0     175     291     444 
   Size:        175     116     153

  Fragments arranged by size: 

                 175     153     116
 
MspA1I CmG'CkG

Cuts at:      0      50     245     444 
   Size:         50     195     199

  Fragments arranged by size: 

                 199     195      50
 
NheI G'CTAG_C

Cuts at:      0      15     444 
   Size:         15     429
 
PvuII CAG'CTG

Cuts at:      0     245     444 
   Size:        245     199
 
SfcI C'TryA_G

Cuts at:      0     315     444 
   Size:        315     129
 
SmaI CCC'GGG

Cuts at:      0     107     444 
   Size:        107     337
 
StuI AGG'CCT

Cuts at:      0     343     444 
   Size:        343     101
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     289     444 
   Size:        289     155
 
XmaI C'CCGG_G

Cuts at:      0     105     444 
   Size:        105     339
 

 Enzymes that do cut:

     AleI     ApoI     AvaI     BanI    BanII     BclI     BmtI   Bpu10I
    BsiEI Bsp1286I    BstXI     BtgI     EaeI     MslI   MspA1I     NheI
    PvuII     SfcI     SmaI     StuI    TaqII     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI