(Linear) (Six-base) MAPSORT of: KIBB6771.seq  Check: 5844  from: 1  to: 354



KIBB6771.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     330     354 
   Size:        330      24
 
AlwNI CAG_nnn'CTG

Cuts at:      0     183     354 
   Size:        183     171
 
BclI T'GATC_A

Cuts at:      0     293     354 
   Size:        293      61
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     225     354 
   Size:        225     129
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     199     354 
   Size:        187      12     155

  Fragments arranged by size: 

                 187     155      12
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     268     354 
   Size:        241      27      86

  Fragments arranged by size: 

                 241      86      27
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     167     354 
   Size:        167     187
 
BsaBI GATnn'nnATC

Cuts at:      0     292     295     354 
   Size:        292       3      59

  Fragments arranged by size: 

                 292      59       3
 
BseYI C'CCAG_C

Cuts at:      0      46     354 
   Size:         46     308
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     224     354 
   Size:         50     174     130

  Fragments arranged by size: 

                 174     130      50
 
BsiEI CG_ry'CG

Cuts at:      0     201     354 
   Size:        201     153
 
BspHI T'CATG_A

Cuts at:      0     140     354 
   Size:        140     214
 
BtgI C'CryG_G

Cuts at:      0      23     354 
   Size:         23     331
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     199     330     354 
   Size:        187      12     131      24

  Fragments arranged by size: 

                 187     131      24      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0     162     224     354 
   Size:        162      62     130

  Fragments arranged by size: 

                 162     130      62
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     354 
   Size:        167     187
 
MslI CAynn'nnrTG

Cuts at:      0     145     354 
   Size:        145     209
 
MspA1I CmG'CkG

Cuts at:      0      19     354 
   Size:         19     335
 
PfoI T'CCnGG_A

Cuts at:      0     164     354 
   Size:        164     190
 
PpuMI rG'GwC_Cy

Cuts at:      0     162     354 
   Size:        162     192
 
PstI C_TGCA'G

Cuts at:      0     104     354 
   Size:        104     250
 
SfcI C'TryA_G

Cuts at:      0     100     354 
   Size:        100     254
 
SmlI C'TyrA_G

Cuts at:      0     256     283     354 
   Size:        256      27      71

  Fragments arranged by size: 

                 256      71      27
 
Tth111I GACn'n_nGTC

Cuts at:      0     160     354 
   Size:        160     194
 

 Enzymes that do cut:

     AcuI    AlwNI     BclI     BmrI     BpmI    BpuEI     BsaI    BsaBI
    BseYI     BsgI    BsiEI    BspHI     BtgI  Eco57MI EcoO109I     MmeI
     MslI   MspA1I     PfoI    PpuMI     PstI     SfcI     SmlI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI