(Linear) (Six-base) MAPSORT of: KIBB6773.seq  Check: 8536  from: 1  to: 354



KIBB6773.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      30     354 
   Size:         30     324
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     354 
   Size:         47     307
 
AflIII A'CryG_T

Cuts at:      0     144     354 
   Size:        144     210
 
AlwNI CAG_nnn'CTG

Cuts at:      0     183     354 
   Size:        183     171
 
BclI T'GATC_A

Cuts at:      0     293     354 
   Size:        293      61
 
BglI GCCn_nnn'nGGC

Cuts at:      0     217     354 
   Size:        217     137
 
BlpI GC'TnA_GC

Cuts at:      0      14     354 
   Size:         14     340
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     354 
   Size:        187     167
 
BsaAI yAC'GTr

Cuts at:      0     145     354 
   Size:        145     209
 
BsaBI GATnn'nnATC

Cuts at:      0     292     354 
   Size:        292      62
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      62     139     354 
   Size:         62      77     215

  Fragments arranged by size: 

                 215      77      62
 
BseYI C'CCAG_C

Cuts at:      0     300     354 
   Size:        300      54
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      30     354 
   Size:         30     324
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     324     354 
   Size:        324      30
 
BtsI GCAGTG_nn'

Cuts at:      0     249     354 
   Size:        249     105
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     187     354 
   Size:         47     140     167

  Fragments arranged by size: 

                 167     140      47
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      26     354 
   Size:         26     328
 
EcoO109I rG'GnC_Cy

Cuts at:      0      58     162     354 
   Size:         58     104     192

  Fragments arranged by size: 

                 192     104      58
 
MslI CAynn'nnrTG

Cuts at:      0     145     322     354 
   Size:        145     177      32

  Fragments arranged by size: 

                 177     145      32
 
PfoI T'CCnGG_A

Cuts at:      0     164     354 
   Size:        164     190
 
PpuMI rG'GwC_Cy

Cuts at:      0      58     162     354 
   Size:         58     104     192

  Fragments arranged by size: 

                 192     104      58
 
SanDI GG'GwC_CC

Cuts at:      0      58     354 
   Size:         58     296
 
SfcI C'TryA_G

Cuts at:      0     100     354 
   Size:        100     254
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     215     354 
   Size:        215     139
 
Tth111I GACn'n_nGTC

Cuts at:      0     160     354 
   Size:        160     194
 

 Enzymes that do cut:

     AarI     AcuI   AflIII    AlwNI     BclI     BglI     BlpI     BpmI
    BsaAI    BsaBI    BseRI    BseYI    BspMI    BstXI     BtsI  Eco57MI
    EcoNI EcoO109I     MslI     PfoI    PpuMI    SanDI     SfcI    TaqII
  Tth111I

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI    BglII Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI
    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     TstI     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI