(Linear) (Six-base) MAPSORT of: KIBB6775.seq  Check: 401  from: 1  to: 354



KIBB6775.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

Acc65I G'GTAC_C

Cuts at:      0     228     354 
   Size:        228     126
 
AgeI A'CCGG_T

Cuts at:      0      76     354 
   Size:         76     278
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     211     354 
   Size:        211     143
 
ApoI r'AATT_y

Cuts at:      0      67     354 
   Size:         67     287
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     212     245     354 
   Size:        212      33     109

  Fragments arranged by size: 

                 212     109      33
 
BanI G'GyrC_C

Cuts at:      0     228     354 
   Size:        228     126
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     354 
   Size:        220     134
 
BsaWI w'CCGG_w

Cuts at:      0      76     354 
   Size:         76     278
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      55      73      76     354 
   Size:         55      18       3     278

  Fragments arranged by size: 

                 278      55      18       3
 
BsiHKAI G_wGCw'C

Cuts at:      0      95     128     354 
   Size:         95      33     226

  Fragments arranged by size: 

                 226      95      33
 
Bsp1286I G_dGCh'C

Cuts at:      0      95     128     354 
   Size:         95      33     226

  Fragments arranged by size: 

                 226      95      33
 
BsrBI CCG'CTC

Cuts at:      0     339     354 
   Size:        339      15
 
BsrFI r'CCGG_y

Cuts at:      0      76     346     354 
   Size:         76     270       8

  Fragments arranged by size: 

                 270      76       8
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     327     354 
   Size:        327      27
 
BtsI GCAGTG_nn'

Cuts at:      0     183     354 
   Size:        183     171
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     354 
   Size:        220     134
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     177     354 
   Size:        177     177
 
HincII GTy'rAC

Cuts at:      0     270     354 
   Size:        270      84
 
KpnI G_GTAC'C

Cuts at:      0     232     354 
   Size:        232     122
 
NaeI GCC'GGC

Cuts at:      0     348     354 
   Size:        348       6
 
NgoMIV G'CCGG_C

Cuts at:      0     346     354 
   Size:        346       8
 
PstI C_TGCA'G

Cuts at:      0      50     354 
   Size:         50     304
 
SfcI C'TryA_G

Cuts at:      0      46     354 
   Size:         46     308
 
StuI AGG'CCT

Cuts at:      0     172     354 
   Size:        172     182
 
Tth111I GACn'n_nGTC

Cuts at:      0     313     354 
   Size:        313      41
 
XmnI GAAnn'nnTTC

Cuts at:      0     172     354 
   Size:        172     182
 

 Enzymes that do cut:

   Acc65I     AgeI     AlfI     ApoI     BaeI     BanI     BpmI    BsaWI
    BseRI  BsiHKAI Bsp1286I    BsrBI    BsrFI    BtgZI     BtsI  Eco57MI
    EcoNI   HincII     KpnI     NaeI   NgoMIV     PstI     SfcI     StuI
  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII    BamHI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StyI     SwaI    TaqII     TatI     TstI     XbaI     XcmI     XhoI
     XmaI     ZraI