(Linear) (Six-base) MAPSORT of: KIBB6777.seq  Check: 4852  from: 1  to: 357



KIBB6777.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     357 
   Size:        199     158
 
ApoI r'AATT_y

Cuts at:      0     120     265     357 
   Size:        120     145      92

  Fragments arranged by size: 

                 145     120      92
 
BmgBI CAC'GTC

Cuts at:      0      94     357 
   Size:         94     263
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     357 
   Size:         73     284
 
Bpu10I CC'TnA_GC

Cuts at:      0      18     357 
   Size:         18     339
 
BsiHKAI G_wGCw'C

Cuts at:      0      29     357 
   Size:         29     328
 
BsmI GAATG_Cn'

Cuts at:      0     210     357 
   Size:        210     147
 
Bsp1286I G_dGCh'C

Cuts at:      0      29     357 
   Size:         29     328
 
BtgI C'CryG_G

Cuts at:      0      11     357 
   Size:         11     346
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     199     357 
   Size:         73     126     158

  Fragments arranged by size: 

                 158     126      73
 
EcoRI G'AATT_C

Cuts at:      0     265     357 
   Size:        265      92
 
HincII GTy'rAC

Cuts at:      0     171     357 
   Size:        171     186
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     357 
   Size:        182     175
 
TatI w'GTAC_w

Cuts at:      0     254     357 
   Size:        254     103
 
Tth111I GACn'n_nGTC

Cuts at:      0     318     357 
   Size:        318      39
 

 Enzymes that do cut:

     AcuI     ApoI    BmgBI     BpmI   Bpu10I  BsiHKAI     BsmI Bsp1286I
     BtgI  Eco57MI    EcoRI   HincII     MmeI     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI