(Linear) (Six-base) MAPSORT of: KIBB6779.seq  Check: 4657  from: 1  to: 357



KIBB6779.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     357 
   Size:        160     197
 
AleI CACnn'nnGTG

Cuts at:      0      91     357 
   Size:         91     266
 
AvrII C'CTAG_G

Cuts at:      0     349     357 
   Size:        349       8
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     222     357 
   Size:        222     135
 
BmgBI CAC'GTC

Cuts at:      0     322     357 
   Size:        322      35
 
BspHI T'CATG_A

Cuts at:      0      33     357 
   Size:         33     324
 
BsrGI T'GTAC_A

Cuts at:      0     156     357 
   Size:        156     201
 
BssSI C'ACGA_G

Cuts at:      0       7     357 
   Size:          7     350
 
Bsu36I CC'TnA_GG

Cuts at:      0     254     357 
   Size:        254     103
 
BtgI C'CryG_G

Cuts at:      0      75     327     357 
   Size:         75     252      30

  Fragments arranged by size: 

                 252      75      30
 
EaeI y'GGCC_r

Cuts at:      0     324     357 
   Size:        324      33
 
EarI CTCTTCn'nnn_

Cuts at:      0     285     297     303     309     333     357 
   Size:        285      12       6       6      24      24

  Fragments arranged by size: 

                 285      24      24      12       6       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     357 
   Size:        160     197
 
EcoO109I rG'GnC_Cy

Cuts at:      0     346     357 
   Size:        346      11
 
MslI CAynn'nnrTG

Cuts at:      0      91     357 
   Size:         91     266
 
MspA1I CmG'CkG

Cuts at:      0     162     357 
   Size:        162     195
 
NcoI C'CATG_G

Cuts at:      0      75     357 
   Size:         75     282
 
PpuMI rG'GwC_Cy

Cuts at:      0     346     357 
   Size:        346      11
 
PvuII CAG'CTG

Cuts at:      0     162     357 
   Size:        162     195
 
StyI C'CwwG_G

Cuts at:      0      75     349     357 
   Size:         75     274       8

  Fragments arranged by size: 

                 274      75       8
 
TatI w'GTAC_w

Cuts at:      0     156     357 
   Size:        156     201
 

 Enzymes that do cut:

     AcuI     AleI    AvrII    BciVI    BmgBI    BspHI    BsrGI    BssSI
   Bsu36I     BtgI     EaeI     EarI  Eco57MI EcoO109I     MslI   MspA1I
     NcoI    PpuMI    PvuII     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI