(Linear) (Six-base) MAPSORT of: KIBB6781.seq  Check: 7590  from: 1  to: 360



KIBB6781.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     115     191     360 
   Size:        115      76     169

  Fragments arranged by size: 

                 169     115      76
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     360 
   Size:        136     224
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      85     117     360 
   Size:         85      32     243

  Fragments arranged by size: 

                 243      85      32
 
BamHI G'GATC_C

Cuts at:      0     267     360 
   Size:        267      93
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     206     240     360 
   Size:        206      34     120

  Fragments arranged by size: 

                 206     120      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     151     360 
   Size:        151     209
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     255     360 
   Size:        255     105
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      12     360 
   Size:         12     348
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     329     359     360 
   Size:        329      30       1

  Fragments arranged by size: 

                 329      30       1
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     337     360 
   Size:        337      23
 
BseYI C'CCAG_C

Cuts at:      0     341     360 
   Size:        341      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     210     360 
   Size:        210     150
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     115     191     360 
   Size:        115      76     169

  Fragments arranged by size: 

                 169     115      76
 
BstYI r'GATC_y

Cuts at:      0     267     360 
   Size:        267      93
 
BtgI C'CryG_G

Cuts at:      0     305     360 
   Size:        305      55
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     247     360 
   Size:        247     113
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     360 
   Size:        136     224
 
EcoO109I rG'GnC_Cy

Cuts at:      0     168     360 
   Size:        168     192
 
HincII GTy'rAC

Cuts at:      0     337     360 
   Size:        337      23
 
SpeI A'CTAG_T

Cuts at:      0     147     360 
   Size:        147     213
 
StyI C'CwwG_G

Cuts at:      0     139     360 
   Size:        139     221
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      78     360 
   Size:         78     282
 
TatI w'GTAC_w

Cuts at:      0     257     360 
   Size:        257     103
 
Tth111I GACn'n_nGTC

Cuts at:      0     247     360 
   Size:        247     113
 

 Enzymes that do cut:

     AarI     AcuI     AloI    BamHI     BcgI    BciVI     BmrI     BsaI
    BsaXI    BseRI    BseYI     BsgI    BspMI    BstYI     BtgI     DrdI
  Eco57MI EcoO109I   HincII     SpeI     StyI    TaqII     TatI  Tth111I

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI    BbvCI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SphI     SrfI     SspI     StuI     SwaI     TstI     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI