(Linear) (Six-base) MAPSORT of: KIBB6782.seq  Check: 3068  from: 1  to: 459



KIBB6782.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     459 
   Size:        211     248
 
AflII C'TTAA_G

Cuts at:      0     297     459 
   Size:        297     162
 
ApaLI G'TGCA_C

Cuts at:      0      59     459 
   Size:         59     400
 
ApoI r'AATT_y

Cuts at:      0     270     406     459 
   Size:        270     136      53

  Fragments arranged by size: 

                 270     136      53
 
AseI AT'TA_AT

Cuts at:      0     143     459 
   Size:        143     316
 
Bme1580I G_kGCm'C

Cuts at:      0      63     459 
   Size:         63     396
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     277     459 
   Size:        121     156     182

  Fragments arranged by size: 

                 182     156     121
 
BsiHKAI G_wGCw'C

Cuts at:      0      63     459 
   Size:         63     396
 
Bsp1286I G_dGCh'C

Cuts at:      0      63     459 
   Size:         63     396
 
BsrDI GCAATG_nn'

Cuts at:      0      26     459 
   Size:         26     433
 
BsrGI T'GTAC_A

Cuts at:      0       2     459 
   Size:          2     457
 
BstYI r'GATC_y

Cuts at:      0     352     459 
   Size:        352     107
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     211     277     459 
   Size:        121      90      66     182

  Fragments arranged by size: 

                 182     121      90      66
 
MslI CAynn'nnrTG

Cuts at:      0     172     369     459 
   Size:        172     197      90

  Fragments arranged by size: 

                 197     172      90
 
NspI r_CATG'y

Cuts at:      0     198     459 
   Size:        198     261
 
PasI CC'CwG_GG

Cuts at:      0     305     459 
   Size:        305     154
 
SmlI C'TyrA_G

Cuts at:      0     297     459 
   Size:        297     162
 
TatI w'GTAC_w

Cuts at:      0       2     459 
   Size:          2     457
 
XbaI T'CTAG_A

Cuts at:      0     234     459 
   Size:        234     225
 

 Enzymes that do cut:

     AcuI    AflII    ApaLI     ApoI     AseI Bme1580I     BpmI  BsiHKAI
 Bsp1286I    BsrDI    BsrGI    BstYI  Eco57MI     MslI     NspI     PasI
     SmlI     TatI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI
     ZraI