(Linear) (Six-base) MAPSORT of: KIBB6785.seq  Check: 8645  from: 1  to: 375



KIBB6785.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AfeI AGC'GCT

Cuts at:      0     215     375 
   Size:        215     160
 
ApaI G_GGCC'C

Cuts at:      0     164     375 
   Size:        164     211
 
AvaI C'yCGr_G

Cuts at:      0     136     375 
   Size:        136     239
 
BanI G'GyrC_C

Cuts at:      0     202     375 
   Size:        202     173
 
BanII G_rGCy'C

Cuts at:      0     164     375 
   Size:        164     211
 
BglI GCCn_nnn'nGGC

Cuts at:      0     140     260     323     375 
   Size:        140     120      63      52

  Fragments arranged by size: 

                 140     120      63      52
 
Bme1580I G_kGCm'C

Cuts at:      0     164     205     375 
   Size:        164      41     170

  Fragments arranged by size: 

                 170     164      41
 
BmgBI CAC'GTC

Cuts at:      0     111     375 
   Size:        111     264
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     244     375 
   Size:        244     131
 
BseYI C'CCAG_C

Cuts at:      0      10     186     368     375 
   Size:         10     176     182       7

  Fragments arranged by size: 

                 182     176      10       7
 
Bsp1286I G_dGCh'C

Cuts at:      0     164     205     375 
   Size:        164      41     170

  Fragments arranged by size: 

                 170     164      41
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     248     375 
   Size:        248     127
 
BsrBI CCG'CTC

Cuts at:      0     281     375 
   Size:        281      94
 
BsrFI r'CCGG_y

Cuts at:      0       4     375 
   Size:          4     371
 
EaeI y'GGCC_r

Cuts at:      0       2     261     375 
   Size:          2     259     114

  Fragments arranged by size: 

                 259     114       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     176     375 
   Size:        176     199
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      42     375 
   Size:         42     333
 
FseI GG_CCGG'CC

Cuts at:      0       8     375 
   Size:          8     367
 
FspI TGC'GCA

Cuts at:      0     115     375 
   Size:        115     260
 
HaeII r_GCGC'y

Cuts at:      0      46     217     375 
   Size:         46     171     158

  Fragments arranged by size: 

                 171     158      46
 
MspA1I CmG'CkG

Cuts at:      0     152     290     375 
   Size:        152     138      85

  Fragments arranged by size: 

                 152     138      85
 
NaeI GCC'GGC

Cuts at:      0       6     375 
   Size:          6     369
 
NgoMIV G'CCGG_C

Cuts at:      0       4     375 
   Size:          4     371
 
PasI CC'CwG_GG

Cuts at:      0      78     375 
   Size:         78     297
 
PspOMI G'GGCC_C

Cuts at:      0     160     375 
   Size:        160     215
 
PstI C_TGCA'G

Cuts at:      0     316     375 
   Size:        316      59
 
SapI GCTCTTCn'nnn_

Cuts at:      0     176     375 
   Size:        176     199
 
SfcI C'TryA_G

Cuts at:      0     312     375 
   Size:        312      63
 
SmaI CCC'GGG

Cuts at:      0     138     375 
   Size:        138     237
 
XmaI C'CCGG_G

Cuts at:      0     136     375 
   Size:        136     239
 

 Enzymes that do cut:

     AfeI     ApaI     AvaI     BanI    BanII     BglI Bme1580I    BmgBI
    BseRI    BseYI Bsp1286I    BspMI    BsrBI    BsrFI     EaeI     EarI
     EciI     FseI     FspI    HaeII   MspA1I     NaeI   NgoMIV     PasI
   PspOMI     PstI     SapI     SfcI     SmaI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI    FspAI    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI