(Linear) (Six-base) MAPSORT of: KIBB6786.seq  Check: 154  from: 1  to: 462



KIBB6786.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     346     462 
   Size:        346     116
 
AflIII A'CryG_T

Cuts at:      0     240     462 
   Size:        240     222
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      64      96     462 
   Size:         64      32     366

  Fragments arranged by size: 

                 366      64      32
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     286     462 
   Size:        286     176
 
BbvCI CC'TCA_GC

Cuts at:      0     287     462 
   Size:        287     175
 
BglI GCCn_nnn'nGGC

Cuts at:      0     436     462 
   Size:        436      26
 
BlpI GC'TnA_GC

Cuts at:      0     101     462 
   Size:        101     361
 
BmgBI CAC'GTC

Cuts at:      0      92     462 
   Size:         92     370
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     272     462 
   Size:        272     190
 
Bpu10I CC'TnA_GC

Cuts at:      0     287     443     455     462 
   Size:        287     156      12       7

  Fragments arranged by size: 

                 287     156      12       7
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     462 
   Size:         37     425
 
BsaAI yAC'GTr

Cuts at:      0     243     462 
   Size:        243     219
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      70     100     462 
   Size:         70      30     362

  Fragments arranged by size: 

                 362      70      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     248     462 
   Size:        248     214
 
Bsp1286I G_dGCh'C

Cuts at:      0     248     462 
   Size:        248     214
 
BsrDI GCAATG_nn'

Cuts at:      0      95     462 
   Size:         95     367
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     129     339     462 
   Size:        129     210     123

  Fragments arranged by size: 

                 210     129     123
 
ClaI AT'CG_AT

Cuts at:      0     251     462 
   Size:        251     211
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      78     113     462 
   Size:         78      35     349

  Fragments arranged by size: 

                 349      78      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     365     462 
   Size:        365      97
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     346     462 
   Size:        346     116
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     321     462 
   Size:        321     141
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      70     102     462 
   Size:         70      32     360

  Fragments arranged by size: 

                 360      70      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     375     462 
   Size:        375      87
 
MslI CAynn'nnrTG

Cuts at:      0     253     397     462 
   Size:        253     144      65

  Fragments arranged by size: 

                 253     144      65
 
MspA1I CmG'CkG

Cuts at:      0      63     286     462 
   Size:         63     223     176

  Fragments arranged by size: 

                 223     176      63
 
PasI CC'CwG_GG

Cuts at:      0     217     432     462 
   Size:        217     215      30

  Fragments arranged by size: 

                 217     215      30
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      75     462 
   Size:         75     387
 
PmlI CAC'GTG

Cuts at:      0     243     462 
   Size:        243     219
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     230     262     462 
   Size:        230      32     200

  Fragments arranged by size: 

                 230     200      32
 
PvuII CAG'CTG

Cuts at:      0      63     286     462 
   Size:         63     223     176

  Fragments arranged by size: 

                 223     176      63
 
SapI GCTCTTCn'nnn_

Cuts at:      0     365     462 
   Size:        365      97
 
SmlI C'TyrA_G

Cuts at:      0      52     462 
   Size:         52     410
 
StyI C'CwwG_G

Cuts at:      0     137     296     462 
   Size:        137     159     166

  Fragments arranged by size: 

                 166     159     137
 

 Enzymes that do cut:

     AcuI   AflIII     AloI     BbsI    BbvCI     BglI     BlpI    BmgBI
     BmrI   Bpu10I    BpuEI    BsaAI    BsaXI  BsiHKAI Bsp1286I    BsrDI
    BstXI     ClaI    CspCI     EarI  Eco57MI     FalI    Hin4I     MmeI
     MslI   MspA1I     PasI    PflMI     PmlI     PpiI    PvuII     SapI
     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI    BfrBI    BglII Bme1580I     BmtI
     BplI     BpmI     BsaI    BsaBI    BsaHI    BsaWI    BseRI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI     PfoI     PmeI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI