(Linear) (Six-base) MAPSORT of: KIBB6790.seq  Check: 1459  from: 1  to: 465



KIBB6790.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     465 
   Size:         26     439
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     200     465 
   Size:        200     265
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     280     312     465 
   Size:        280      32     153

  Fragments arranged by size: 

                 280     153      32
 
BamHI G'GATC_C

Cuts at:      0     116     465 
   Size:        116     349
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     331     465 
   Size:         41     290     134

  Fragments arranged by size: 

                 290     134      41
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     465 
   Size:        361     104
 
BsaWI w'CCGG_w

Cuts at:      0     123     465 
   Size:        123     342
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      73     328     465 
   Size:         73     255     137

  Fragments arranged by size: 

                 255     137      73
 
BspEI T'CCGG_A

Cuts at:      0     123     465 
   Size:        123     342
 
BstYI r'GATC_y

Cuts at:      0     116     465 
   Size:        116     349
 
Bsu36I CC'TnA_GG

Cuts at:      0     112     465 
   Size:        112     353
 
BtgI C'CryG_G

Cuts at:      0     242     465 
   Size:        242     223
 
EaeI y'GGCC_r

Cuts at:      0     228     239     420     465 
   Size:        228      11     181      45

  Fragments arranged by size: 

                 228     181      45      11
 
EarI CTCTTCn'nnn_

Cuts at:      0     462     465 
   Size:        462       3
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26      41     331     465 
   Size:         26      15     290     134

  Fragments arranged by size: 

                 290     134      26      15
 
MscI TGG'CCA

Cuts at:      0     230     241     422     465 
   Size:        230      11     181      43

  Fragments arranged by size: 

                 230     181      43      11
 
MspA1I CmG'CkG

Cuts at:      0     174     465 
   Size:        174     291
 
NcoI C'CATG_G

Cuts at:      0     242     465 
   Size:        242     223
 
NspI r_CATG'y

Cuts at:      0     352     465 
   Size:        352     113
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     237     465 
   Size:        237     228
 
PfoI T'CCnGG_A

Cuts at:      0     299     465 
   Size:        299     166
 
PstI C_TGCA'G

Cuts at:      0     179     465 
   Size:        179     286
 
PvuII CAG'CTG

Cuts at:      0     174     465 
   Size:        174     291
 
ScaI AGT'ACT

Cuts at:      0     235     465 
   Size:        235     230
 
SfcI C'TryA_G

Cuts at:      0      90     175     465 
   Size:         90      85     290

  Fragments arranged by size: 

                 290      90      85
 
SmlI C'TyrA_G

Cuts at:      0     376     465 
   Size:        376      89
 
SphI G_CATG'C

Cuts at:      0     352     465 
   Size:        352     113
 
StyI C'CwwG_G

Cuts at:      0     242     465 
   Size:        242     223
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      52     465 
   Size:         52     413
 
TatI w'GTAC_w

Cuts at:      0     233     465 
   Size:        233     232
 

 Enzymes that do cut:

     AcuI     AlfI     AloI    BamHI     BpmI    BpuEI    BsaWI    BseRI
    BspEI    BstYI   Bsu36I     BtgI     EaeI     EarI  Eco57MI     MscI
   MspA1I     NcoI     NspI    PflMI     PfoI     PstI    PvuII     ScaI
     SfcI     SmlI     SphI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI