(Linear) (Six-base) MAPSORT of: KIBB6791.seq  Check: 5865  from: 1  to: 381



KIBB6791.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

ApoI r'AATT_y

Cuts at:      0     184     381 
   Size:        184     197
 
BanII G_rGCy'C

Cuts at:      0      83     381 
   Size:         83     298
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     348     381 
   Size:        348      33
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     381 
   Size:        232     149
 
BseYI C'CCAG_C

Cuts at:      0     160     381 
   Size:        160     221
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     381 
   Size:        145     236
 
BsiHKAI G_wGCw'C

Cuts at:      0      83     381 
   Size:         83     298
 
Bsp1286I G_dGCh'C

Cuts at:      0      83     381 
   Size:         83     298
 
BspHI T'CATG_A

Cuts at:      0      53     381 
   Size:         53     328
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     342     381 
   Size:        342      39
 
EarI CTCTTCn'nnn_

Cuts at:      0      71     381 
   Size:         71     310
 
EcoICRI GAG'CTC

Cuts at:      0      81     381 
   Size:         81     300
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     381 
   Size:        232     149
 
EcoRI G'AATT_C

Cuts at:      0     184     381 
   Size:        184     197
 
EcoRV GAT'ATC

Cuts at:      0     102     381 
   Size:        102     279
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     233     381 
   Size:         95     138     148

  Fragments arranged by size: 

                 148     138      95
 
MspA1I CmG'CkG

Cuts at:      0     160     381 
   Size:        160     221
 
PstI C_TGCA'G

Cuts at:      0      29     381 
   Size:         29     352
 
SacI G_AGCT'C

Cuts at:      0      83     381 
   Size:         83     298
 
SapI GCTCTTCn'nnn_

Cuts at:      0      71     381 
   Size:         71     310
 
SfcI C'TryA_G

Cuts at:      0      25     381 
   Size:         25     356
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      55     319     334     381 
   Size:         55     264      15      47

  Fragments arranged by size: 

                 264      55      47      15
 
Tth111I GACn'n_nGTC

Cuts at:      0     352     381 
   Size:        352      29
 
XmnI GAAnn'nnTTC

Cuts at:      0     139     381 
   Size:        139     242
 

 Enzymes that do cut:

     ApoI    BanII     BbsI     BpmI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspHI    BstXI     EarI  EcoICRI  Eco57MI    EcoRI    EcoRV     MmeI
   MspA1I     PstI     SacI     SapI     SfcI    TaqII  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI    EcoNI EcoO109I     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI     XbaI
     XcmI     XhoI     XmaI     ZraI