Restriction map for pF1KB6793
 (Linear) (Six-base) MAPSORT of: KIBB6793.seq  Check: 4253  from: 1  to: 381



KIBB6793.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

BbsI GAAGACnn'nnnn_

Cuts at:      0     100     178     381 
   Size:        100      78     203

  Fragments arranged by size: 

                 203     100      78
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     318     381 
   Size:        318      63
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     262     373     381 
   Size:        262     111       8

  Fragments arranged by size: 

                 262     111       8
 
BsrGI T'GTAC_A

Cuts at:      0     144     381 
   Size:        144     237
 
BstEII G'GTnAC_C

Cuts at:      0     267     381 
   Size:        267     114
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     243     381 
   Size:        243     138
 
BtgI C'CryG_G

Cuts at:      0     242     381 
   Size:        242     139
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      12     246     381 
   Size:         12     234     135

  Fragments arranged by size: 

                 234     135      12
 
EaeI y'GGCC_r

Cuts at:      0     164     230     381 
   Size:        164      66     151

  Fragments arranged by size: 

                 164     151      66
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     268     381 
   Size:        268     113
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      36     312     381 
   Size:         36     276      69

  Fragments arranged by size: 

                 276      69      36
 
FspI TGC'GCA

Cuts at:      0     286     381 
   Size:        286      95
 
HincII GTy'rAC

Cuts at:      0     222     381 
   Size:        222     159
 
MscI TGG'CCA

Cuts at:      0     232     381 
   Size:        232     149
 
MslI CAynn'nnrTG

Cuts at:      0     115     381 
   Size:        115     266
 
MspA1I CmG'CkG

Cuts at:      0      88     381 
   Size:         88     293
 
PvuII CAG'CTG

Cuts at:      0      88     381 
   Size:         88     293
 
SmlI C'TyrA_G

Cuts at:      0     297     381 
   Size:        297      84
 
StyI C'CwwG_G

Cuts at:      0      32      98     131     176     381 
   Size:         32      66      33      45     205

  Fragments arranged by size: 

                 205      66      45      33      32
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      37     381 
   Size:         37     344
 
TatI w'GTAC_w

Cuts at:      0     144     381 
   Size:        144     237
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      39     240     381 
   Size:         39     201     141

  Fragments arranged by size: 

                 201     141      39
 
XmnI GAAnn'nnTTC

Cuts at:      0      19     381 
   Size:         19     362
 

 Enzymes that do cut:

     BbsI    BpuEI    BseRI    BsrGI   BstEII    BstXI     BtgI    BtgZI
     EaeI     EciI     FalI     FspI   HincII     MscI     MslI   MspA1I
    PvuII     SmlI     StyI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI    FspAI    HaeII    Hin4I
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com