(Linear) (Six-base) MAPSORT of: KIBB6793.seq Check: 4253 from: 1 to: 381 KIBB6793.orf With 182 enzymes: SgfI * June 26, 2008 10:48 .. BbsI GAAGACnn'nnnn_ Cuts at: 0 100 178 381 Size: 100 78 203 Fragments arranged by size: 203 100 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 318 381 Size: 318 63 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 262 373 381 Size: 262 111 8 Fragments arranged by size: 262 111 8 BsrGI T'GTAC_A Cuts at: 0 144 381 Size: 144 237 BstEII G'GTnAC_C Cuts at: 0 267 381 Size: 267 114 BstXI CCAn_nnnn'nTGG Cuts at: 0 243 381 Size: 243 138 BtgI C'CryG_G Cuts at: 0 242 381 Size: 242 139 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 12 246 381 Size: 12 234 135 Fragments arranged by size: 234 135 12 EaeI y'GGCC_r Cuts at: 0 164 230 381 Size: 164 66 151 Fragments arranged by size: 164 151 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 268 381 Size: 268 113 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 36 312 381 Size: 36 276 69 Fragments arranged by size: 276 69 36 FspI TGC'GCA Cuts at: 0 286 381 Size: 286 95 HincII GTy'rAC Cuts at: 0 222 381 Size: 222 159 MscI TGG'CCA Cuts at: 0 232 381 Size: 232 149 MslI CAynn'nnrTG Cuts at: 0 115 381 Size: 115 266 MspA1I CmG'CkG Cuts at: 0 88 381 Size: 88 293 PvuII CAG'CTG Cuts at: 0 88 381 Size: 88 293 SmlI C'TyrA_G Cuts at: 0 297 381 Size: 297 84 StyI C'CwwG_G Cuts at: 0 32 98 131 176 381 Size: 32 66 33 45 205 Fragments arranged by size: 205 66 45 33 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 37 381 Size: 37 344 TatI w'GTAC_w Cuts at: 0 144 381 Size: 144 237 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 39 240 381 Size: 39 201 141 Fragments arranged by size: 201 141 39 XmnI GAAnn'nnTTC Cuts at: 0 19 381 Size: 19 362 Enzymes that do cut: BbsI BpuEI BseRI BsrGI BstEII BstXI BtgI BtgZI EaeI EciI FalI FspI HincII MscI MslI MspA1I PvuII SmlI StyI TaqII TatI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspAI HaeII Hin4I HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI ZraI