Restriction map for pF1KB6795
 (Linear) (Six-base) MAPSORT of: KIBB6795.seq  Check: 7348  from: 1  to: 384



KIBB6795.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     178     384 
   Size:        178     206
 
AccI GT'mk_AC

Cuts at:      0       7     384 
   Size:          7     377
 
AflIII A'CryG_T

Cuts at:      0     248     384 
   Size:        248     136
 
AlwNI CAG_nnn'CTG

Cuts at:      0     109     384 
   Size:        109     275
 
AvaI C'yCGr_G

Cuts at:      0      46     384 
   Size:         46     338
 
BfrBI ATG'CAT

Cuts at:      0      87     384 
   Size:         87     297
 
Bme1580I G_kGCm'C

Cuts at:      0     268     295     384 
   Size:        268      27      89

  Fragments arranged by size: 

                 268      89      27
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     142     326     384 
   Size:        142     184      58

  Fragments arranged by size: 

                 184     142      58
 
BsaAI yAC'GTr

Cuts at:      0     269     384 
   Size:        269     115
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     217     384 
   Size:        217     167
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     384 
   Size:        332      52
 
Bsp1286I G_dGCh'C

Cuts at:      0     268     295     384 
   Size:        268      27      89

  Fragments arranged by size: 

                 268      89      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     178     384 
   Size:        178     206
 
BsrDI GCAATG_nn'

Cuts at:      0      98     182     384 
   Size:         98      84     202

  Fragments arranged by size: 

                 202      98      84
 
BsrFI r'CCGG_y

Cuts at:      0     254     384 
   Size:        254     130
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     168     352     384 
   Size:        168     184      32

  Fragments arranged by size: 

                 184     168      32
 
DraIII CAC_nnn'GTG

Cuts at:      0      32     384 
   Size:         32     352
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     178     384 
   Size:        178     206
 
EaeI y'GGCC_r

Cuts at:      0     130     206     384 
   Size:        130      76     178

  Fragments arranged by size: 

                 178     130      76
 
EarI CTCTTCn'nnn_

Cuts at:      0     235     384 
   Size:        235     149
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     113     384 
   Size:        113     271
 
HincII GTy'rAC

Cuts at:      0       8     384 
   Size:          8     376
 
MscI TGG'CCA

Cuts at:      0     208     384 
   Size:        208     176
 
MslI CAynn'nnrTG

Cuts at:      0      82     166     278     384 
   Size:         82      84     112     106

  Fragments arranged by size: 

                 112     106      84      82
 
NsiI A_TGCA'T

Cuts at:      0      89     384 
   Size:         89     295
 
NspI r_CATG'y

Cuts at:      0     252     384 
   Size:        252     132
 
PciI A'CATG_T

Cuts at:      0     248     384 
   Size:        248     136
 
PspXI vC'TCGA_Gb

Cuts at:      0      46     384 
   Size:         46     338
 
PstI C_TGCA'G

Cuts at:      0     329     384 
   Size:        329      55
 
SalI G'TCGA_C

Cuts at:      0       6     384 
   Size:          6     378
 
SbfI CC_TGCA'GG

Cuts at:      0     329     384 
   Size:        329      55
 
ScaI AGT'ACT

Cuts at:      0     376     384 
   Size:        376       8
 
SfcI C'TryA_G

Cuts at:      0     325     384 
   Size:        325      59
 
SmlI C'TyrA_G

Cuts at:      0      46     384 
   Size:         46     338
 
StyI C'CwwG_G

Cuts at:      0     287     384 
   Size:        287      97
 
TatI w'GTAC_w

Cuts at:      0     374     384 
   Size:        374      10
 
Tth111I GACn'n_nGTC

Cuts at:      0     175     384 
   Size:        175     209
 
XhoI C'TCGA_G

Cuts at:      0      46     384 
   Size:         46     338
 

 Enzymes that do cut:

     AarI     AccI   AflIII    AlwNI     AvaI    BfrBI Bme1580I     BsaI
    BsaAI    BseRI     BsgI Bsp1286I    BspMI    BsrDI    BsrFI    BstXI
   DraIII     DrdI     EaeI     EarI    EcoNI   HincII     MscI     MslI
     NsiI     NspI     PciI    PspXI     PstI     SalI     SbfI     ScaI
     SfcI     SmlI     StyI     TatI  Tth111I     XhoI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     EagI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
    SanDI     SapI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI     XbaI     XcmI     XmaI     XmnI     ZraI
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