(Linear) (Six-base) MAPSORT of: KIBB6795.seq Check: 7348 from: 1 to: 384 KIBB6795.orf With 182 enzymes: SgfI * June 26, 2008 10:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 178 384 Size: 178 206 AccI GT'mk_AC Cuts at: 0 7 384 Size: 7 377 AflIII A'CryG_T Cuts at: 0 248 384 Size: 248 136 AlwNI CAG_nnn'CTG Cuts at: 0 109 384 Size: 109 275 AvaI C'yCGr_G Cuts at: 0 46 384 Size: 46 338 BfrBI ATG'CAT Cuts at: 0 87 384 Size: 87 297 Bme1580I G_kGCm'C Cuts at: 0 268 295 384 Size: 268 27 89 Fragments arranged by size: 268 89 27 BsaI GGTCTCn'nnnn_ Cuts at: 0 142 326 384 Size: 142 184 58 Fragments arranged by size: 184 142 58 BsaAI yAC'GTr Cuts at: 0 269 384 Size: 269 115 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 217 384 Size: 217 167 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 332 384 Size: 332 52 Bsp1286I G_dGCh'C Cuts at: 0 268 295 384 Size: 268 27 89 Fragments arranged by size: 268 89 27 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 178 384 Size: 178 206 BsrDI GCAATG_nn' Cuts at: 0 98 182 384 Size: 98 84 202 Fragments arranged by size: 202 98 84 BsrFI r'CCGG_y Cuts at: 0 254 384 Size: 254 130 BstXI CCAn_nnnn'nTGG Cuts at: 0 168 352 384 Size: 168 184 32 Fragments arranged by size: 184 168 32 DraIII CAC_nnn'GTG Cuts at: 0 32 384 Size: 32 352 DrdI GACnn_nn'nnGTC Cuts at: 0 178 384 Size: 178 206 EaeI y'GGCC_r Cuts at: 0 130 206 384 Size: 130 76 178 Fragments arranged by size: 178 130 76 EarI CTCTTCn'nnn_ Cuts at: 0 235 384 Size: 235 149 EcoNI CCTnn'n_nnAGG Cuts at: 0 113 384 Size: 113 271 HincII GTy'rAC Cuts at: 0 8 384 Size: 8 376 MscI TGG'CCA Cuts at: 0 208 384 Size: 208 176 MslI CAynn'nnrTG Cuts at: 0 82 166 278 384 Size: 82 84 112 106 Fragments arranged by size: 112 106 84 82 NsiI A_TGCA'T Cuts at: 0 89 384 Size: 89 295 NspI r_CATG'y Cuts at: 0 252 384 Size: 252 132 PciI A'CATG_T Cuts at: 0 248 384 Size: 248 136 PspXI vC'TCGA_Gb Cuts at: 0 46 384 Size: 46 338 PstI C_TGCA'G Cuts at: 0 329 384 Size: 329 55 SalI G'TCGA_C Cuts at: 0 6 384 Size: 6 378 SbfI CC_TGCA'GG Cuts at: 0 329 384 Size: 329 55 ScaI AGT'ACT Cuts at: 0 376 384 Size: 376 8 SfcI C'TryA_G Cuts at: 0 325 384 Size: 325 59 SmlI C'TyrA_G Cuts at: 0 46 384 Size: 46 338 StyI C'CwwG_G Cuts at: 0 287 384 Size: 287 97 TatI w'GTAC_w Cuts at: 0 374 384 Size: 374 10 Tth111I GACn'n_nGTC Cuts at: 0 175 384 Size: 175 209 XhoI C'TCGA_G Cuts at: 0 46 384 Size: 46 338 Enzymes that do cut: AarI AccI AflIII AlwNI AvaI BfrBI Bme1580I BsaI BsaAI BseRI BsgI Bsp1286I BspMI BsrDI BsrFI BstXI DraIII DrdI EaeI EarI EcoNI HincII MscI MslI NsiI NspI PciI PspXI PstI SalI SbfI ScaI SfcI SmlI StyI TatI Tth111I XhoI Enzymes that do not cut: AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI EagI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PvuI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TstI XbaI XcmI XmaI XmnI ZraI