(Linear) (Six-base) MAPSORT of: KIBB6801.seq  Check: 2840  from: 1  to: 474



KIBB6801.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AccI GT'mk_AC

Cuts at:      0     368     474 
   Size:        368     106
 
Acc65I G'GTAC_C

Cuts at:      0     101     474 
   Size:        101     373
 
AlwNI CAG_nnn'CTG

Cuts at:      0     322     474 
   Size:        322     152
 
BanI G'GyrC_C

Cuts at:      0     101     474 
   Size:        101     373
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     361     395     474 
   Size:        361      34      79

  Fragments arranged by size: 

                 361      79      34
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     294     474 
   Size:        294     180
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     407     474 
   Size:        407      67
 
BsaBI GATnn'nnATC

Cuts at:      0     318     474 
   Size:        318     156
 
BstBI TT'CG_AA

Cuts at:      0     170     474 
   Size:        170     304
 
BstYI r'GATC_y

Cuts at:      0     197     319     474 
   Size:        197     122     155

  Fragments arranged by size: 

                 197     155     122
 
Bsu36I CC'TnA_GG

Cuts at:      0     397     474 
   Size:        397      77
 
EarI CTCTTCn'nnn_

Cuts at:      0     452     474 
   Size:        452      22
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     398     474 
   Size:        398      76
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     189     221     474 
   Size:        189      32     253

  Fragments arranged by size: 

                 253     189      32
 
HincII GTy'rAC

Cuts at:      0     445     474 
   Size:        445      29
 
KpnI G_GTAC'C

Cuts at:      0     105     474 
   Size:        105     369
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     215     474 
   Size:        215     259
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     438     474 
   Size:        438      36
 
PfoI T'CCnGG_A

Cuts at:      0     239     474 
   Size:        239     235
 
PshAI GACnn'nnGTC

Cuts at:      0     440     474 
   Size:        440      34
 
SpeI A'CTAG_T

Cuts at:      0     363     474 
   Size:        363     111
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      99     474 
   Size:         99     375
 
XmnI GAAnn'nnTTC

Cuts at:      0     176     474 
   Size:        176     298
 

 Enzymes that do cut:

     AccI   Acc65I    AlwNI     BanI     BcgI     BplI     BsaI    BsaBI
    BstBI    BstYI   Bsu36I     EarI     FalI    Hin4I   HincII     KpnI
     MmeI    PflMI     PfoI    PshAI     SpeI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI